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Table 1.6 R0 values for some Alexa Fluor dyes.

Donor
Acceptor
Alexa Fluor 488Alexa Fluor 546Alexa Fluor 555Alexa Fluor 568Alexa Fluor 594Alexa Fluor 647
Alexa Fluor 35050     
Alexa Fluor 488NA6470626056
Alexa Fluor 546 NA 707174
Alexa Fluor 555  NA 4751
Alexa Fluor 568   NA 82
Alexa Fluor 594    NA85
Alexa Fluor 647     NA
R0 values in angstroms (Å) represent the distance at which fluorescence resonance energy transfer from the donor dye to the acceptor dye is 50% efficient (Förster radius). Values were calculated from spectroscopic data as outlined (Fluorescence Resonance Energy Transfer (FRET)—Note 1.2). NA = Not applicable.

For Research Use Only. Not for use in diagnostic procedures.