Table 20.4—Reactive pH indicator dyes.

pH Indicator Preferred Reactive Form
BCECFBCECF (B1151, Probes Useful at Near-Neutral pH—Section 20.2) *
Carboxyfluorescein5-(and 6-)carboxyfluorescein, succinimidyl ester (C1311, Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5)
6-JOE6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester (C6171MP, Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5)
Naphthofluorescein5-(and 6-)carboxynaphthofluorescein, succinimidyl ester (C653)
Oregon Green 488Oregon Green 488 carboxylic acid, succinimidyl ester (O6147, O6149)
Oregon Green 514Oregon Green 514 carboxylic acid, succinimidyl ester (O6139)
pHrodopHrodo succinimidyl ester (P36600)
SNARF-1SNARF-1 carboxylic acid, acetate, succinimidyl ester (S22801)
5-(and 6-)carboxy SNARF-1 (C1270, Probes Useful at Near-Neutral pH—Section 20.2) *
SNARF-4FSNARF-4F carboxylic acid (S23920, Probes Useful at Near-Neutral pH—Section 20.2) *
SNARF-5FSNARF-5F carboxylic acid (S23922, Probes Useful at Near-Neutral pH—Section 20.2) *
* Carboxylic acids require activation with EDAC/NHSS before reaction with amines (Introduction to Amine Modification—Section 1.1).

For Research Use Only. Not for use in diagnostic procedures.