NovaFluor Blue 660-120S excitation shown as dotted line and emission shown as solid red histogram
3488660/20492665(in buffer) 5
flow cytometry

Invitrogen NovaFluor Blue 660 dyes are available with two different levels of brightness—NovaFluor Blue 660-40S and NovaFluor Blue 660-120S—that allow differential matching of the dye brightness to the antigen density or abundance without panel redesign. NovaFluor Blue 660-120S dye is the brighter dye. NovaFluor Blue 660-120S dye can be used to replace PE-tandem dyes, such as PE-Cy5 and PE/Fire™ 640, using the 488 nm blue laser line for excitation. Because PE-tandem dyes are cross-excited by both blue and yellow-green lasers, the replacement of these dyes with NovaFluor Blue 660-120S dye can allow detection of an additional marker labeled with, for example, NovaFluor Yellow 660 dye.

The macromolecule-based NovaFluor Blue 660-120S dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.   

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.    

Search NovaFluor Blue 660-120S primary antibodies

Benefits of NovaFluor Blue 660-120S dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Higher intensity (~3 times brighter than NovaFluor Blue 660-40S dye) allows it to be paired with medium-to-low abundance antigens
  • Can serve as a replacement for PE-tandem dyes using the blue laser, with reduced cross-laser excitation and fluorescent spillover
  • Brighter replacement for PerCP-Cy5.5
  • Single PE-tandem dyes can be replaced by the NovaFluor Blue 660-120S and NovaFluor Yellow 660 dyes to allow detection of an additional marker in a flow cytometry panel
  • NovaFluor Blue 660-40S and NovaFluor Blue 660-120S can be used together and unmixed using a spectral flow cytometer 
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Blue 660-120S dye
Spectral signature of NovaFluor Blue 660-120S dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 660-120S were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD19 NovaFluor Blue 660-120S-stained normal human PBMCs
Staining of human peripheral blood cells with NovaFluor Blue 660-120S conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD19 Monoclonal Antibody, NovaFluor Blue 660-120S (right). Data was acquired in the B7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

NovaFluor dyes

NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

Additional resources

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