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2 | 488 | 720/24 | 493 | 726 | (in buffer) 3 | flow cytometry |
Invitrogen NovaFluor Blue 725 dye has a unique emission spectrum when excited by the 488 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution.
NovaFluor Blue 725 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Blue 725 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
Spectral signature of NovaFluor Blue 725 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 725 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of mouse splenocytes with NovaFluor Blue 725 conjugated antibody. C57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, eFluor 450 only (left) or with CD8a Monoclonal Antibody, NovaFluor Blue 725 (right). Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit, for 405 nm excitation.
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标,经许可使用。
仅供科研使用,不可用于诊断目的。