Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.

The MabCaptureC™ High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH enabling efficient cleaning of the chromatography resin
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A product for antibody purification

Product links in the last row of each column

 Dynabeads
Protein A Magnetic Beads		Pierce
Protein A Agarose		Pierce
Protein A Plus Agarose		Pierce
Recombinant Protein A Agarose		MabCaptureC™ High Capacity Protein A, Alkaline-Stable
Bead or resin size2.8 μm45–165 μm45–165 μm45–165 μm75 μm
Binding capacity250–300 μg human IgG/mL beads12–19 mg human IgG/mL resin34 mg human IgG/mL resin15–17 mg human IgG/mL resin58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthrough
Maximum linear flow rateN/A700 cm/hr700 cm/hr700 cm/hr300 cm/hr or less (150 cm/hr recommended)
SupportMonosized, super paramagnetic polystyrene beadsCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseHighly cross-linked agarose
Recommended application scalescreeningbatch, pilotscreening, batchbatchbatch, pilot, process
Formats availableloose beadsloose beads, chromatography cartridgesloose beads, pre-packed spin columns and kits, 96-well filter platesloose beadsloose beads
Order product(s)10001D20333
(loose resin)
88924
(1 mL cartridge)
89925
(5 mL cartridge)
44667
(spin kit)		22810
(loose resin)
45202
(spin plates)		20365A53031, 10 mL 50% slurry
A53033, 50 mL 50% slurry
A53034, 200 mL 50% slurry
1963662250, 250 mL 50% slurry
196366201L, 1 L 50% slurry
196366205L, 5 L 50% slurry
196366210L, 10 L 50% slurry
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