Invitrogen

Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

97 Published Figures

80 References

View all (65) Ly-6G/Ly-6C antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Ly-6G/Ly-6C Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

Ly-6G/Ly-6C Antibody (35-5931-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PE-Cyanine5-5 (Product # 35-4031-80) (left) or 0.06 µg of Anti-Mouse Ly-6G (Gr-1) PE-Cyanine5-5 (right). Cells in the large scatter population were used for analysis.

Product Image Gallery

Product Details

35-5931-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 78 publications 78 publications

Functional Assay (Functional)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Dog, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RB6-8C5

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469740

Product Specific Information

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (Product # 9668) be used when looking at Ly-6G specific targets.

Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

CD8⁺ T cell-intrinsic IL-6 signaling promotes resistance to anti-PD-L1 immunotherapy.

Cell reports. Medicine

Huseni MA,Wang L,Klementowicz JE,Yuen K,Breart B,Orr C,Liu LF,Li Y,Gupta V,Li C,Rishipathak D,Peng J,Şenbabaoǧlu Y,Modrusan Z,Keerthivasan S,Madireddi S,Chen YJ,Fraser EJ,Leng N,Hamidi H,Koeppen H,Ziai J,Hashimoto K,Fassò M,Williams P,McDermott DF,Rosenberg JE,Powles T,Emens LA,Hegde PS,Mellman I,Turley SJ,Wilson MS,Mariathasan S,Molinero L,Merchant M,West NR

35-5931-82 was used in Flow cytometry/Cell sorting to identify interleukin 6 (IL-6) as a correlate of poor response to atezolizumab (anti-PD-L1) in large clinical trials of advanced kidney, breast, and bladder cancers.

Tue Jan 17 00:00:00 EST 2023

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

BRD4-mediated repression of p53 is a target for combination therapy in AML.

Nature communications

Latif AL,Newcombe A,Li S,Gilroy K,Robertson NA,Lei X,Stewart HJS,Cole J,Terradas MT,Rishi L,McGarry L,McKeeve C,Reid C,Clark W,Campos J,Kirschner K,Davis A,Lopez J,Sakamaki JI,Morton JP,Ryan KM,Tait SWG,Abraham SA,Holyoake T,Higgins B,Huang X,Blyth K,Copland M,Chevassut TJT,Keeshan K,Adams PD

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Jan 11 00:00:00 EST 2021

Design, Synthesis and Biological Evaluation of Arylpyridin-2-yl Guanidine Derivatives and Cyclic Mimetics as Novel MSK1 Inhibitors. An Application in an Asthma Model.

Molecules (Basel, Switzerland)

Bollenbach M,Nemska S,Wagner P,Camelin G,Daubeuf F,Obrecht A,Villa P,Rognan D,Bihel F,Bourguignon JJ,Schmitt M,Frossard N

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Jan 13 00:00:00 EST 2021

IFNγ-Producing γ/δ T Cells Accumulate in the Fetal Brain Following Intrauterine Inflammation.

Frontiers in immunology

Lewis EL,Tulina N,Anton L,Brown AG,Porrett PM,Elovitz MA

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Jan 05 00:00:00 EST 2022

Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3.

Nature communications

Zheng Z,Li YN,Jia S,Zhu M,Cao L,Tao M,Jiang J,Zhan S,Chen Y,Gao PJ,Hu W,Wang Y,Shao C,Shi Y

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Retinoic Acid Inhibits Tumor-Associated Mesenchymal Stromal Cell Transformation in Melanoma.

Frontiers in cell and developmental biology

Lou Q,Zhao M,Xu Q,Xie S,Liang Y,Chen J,Yuan L,Wang L,Jiang L,Mou L,Lin D,Zhao M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sat Apr 24 00:00:00 EDT 2021

Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201.

PloS one

Trenevska I,Anderson AP,Bentley C,Hassanali T,Wiblin S,Maguire S,Pezzella F,Banham AH,Li D

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Sep 29 00:00:00 EDT 2021

Abraxane-induced bone marrow CD11b⁺ myeloid cell depletion in tumor-bearing mice is visualized by μPET-CT with ⁶⁴Cu-labeled anti-CD11b and prevented by anti-CSF-1.

Theranostics

Cao Q,Huang Q,Wang YA,Li C

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Jul 23 00:00:00 EDT 2021

Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

Science advances

Abu El Maaty MA,Grelet E,Keime C,Rerra AI,Gantzer J,Emprou C,Terzic J,Lutzing R,Bornert JM,Laverny G,Metzger D

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist.

eLife

Glassman CR,Su L,Majri-Morrison SS,Winkelmann H,Mo F,Li P,Pérez-Cruz M,Ho PP,Koliesnik I,Nagy N,Hnizdilova T,Picton LK,Kovar M,Bollyky P,Steinman L,Meyer E,Piehler J,Leonard WJ,Garcia KC

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Plaque-associated myeloid cells derive from resident microglia in an Alzheimer's disease model.

The Journal of experimental medicine

Reed-Geaghan EG,Croxford AL,Becher B,Landreth GE

35-5931 was used in Flow cytometry/Cell sorting to genetically label different myeloid populations and unequivocally demonstrate that plaque-associated myeloid cells in the AD brain are derived exclusively from resident microglia.

Mon Apr 06 00:00:00 EDT 2020

In Situ Therapeutic Cancer Vaccination with an Oncolytic Virus Expressing Membrane-Tethered IL-2.

Molecular therapy oncolytics

Liu W,Dai E,Liu Z,Ma C,Guo ZS,Bartlett DL

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Deficiency of immunoglobulin E protects mice from experimental abdominal aortic aneurysms.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Deng Z,Zhang X,Liu F,Yang C,Shi GP

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Flagellin-Stimulated Production of Interferon-β Promotes Anti-Flagellin IgG2c and IgA Responses.

Molecules and cells

Kang W,Park A,Huh JW,You G,Jung DJ,Song M,Lee HK,Kim YM

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Effects of Hypertonic Saline and Hydroxyethyl Starch on Myeloid-Derived Suppressor Cells in Hemorrhagic Shock Mice under Secondary Bacterial Attack.

BioMed research international

Jiang JK,Hong LJ,Lu YQ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Long Non-Coding RNA Hotairm1 Promotes S100A9 Support of MDSC Expansion during Sepsis.

Journal of clinical & cellular immunology

Alkhateeb T,Bah I,Kumbhare A,Youssef D,Yao ZQ,McCall CE,Gazzar ME

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sat Aug 13 00:00:00 EDT 2022

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection.

Cell reports

Dokoshi T,Zhang LJ,Li F,Nakatsuji T,Butcher A,Yoshida H,Shimoda M,Okada Y,Gallo RL

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Bacterial and Fungal Toll-Like Receptor Activation Elicits Type I IFN Responses in Mast Cells.

Frontiers in immunology

Kornstädt L,Pierre S,Weigert A,Ebersberger S,Schäufele TJ,Kolbinger A,Schmid T,Cohnen J,Thomas D,Ferreirós N,Brüne B,Ebersberger I,Scholich K

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Sensing and signaling of immunogenic extracellular RNAs restrain group 2 innate lymphoid cell-driven acute lung inflammation and airway hyperresponsiveness.

PloS one

She L,Alanazi HH,Yan L,Brooks EG,Dube PH,Xiang Y,Zhang F,Sun Y,Liu Y,Zhang X,Li XD

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Sep 24 00:00:00 EDT 2020

Protocol for Flow Cytometric Detection of Immune Cell Infiltration in the Epidermis and Dermis of a Psoriasis Mouse Model.

STAR protocols

Lou F,Sun Y,Wang H

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Dec 18 00:00:00 EST 2020

Neutrophils contribute to spontaneous resolution of liver inflammation and fibrosis via microRNA-223.

The Journal of clinical investigation

Calvente CJ,Tameda M,Johnson CD,Del Pilar H,Lin YC,Adronikou N,De Mollerat Du Jeu X,Llorente C,Boyer J,Feldstein AE

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Jul 11 00:00:00 EDT 2019

Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection.

Cell death discovery

Wang N,Yang X,Sun J,Sun Z,Ma Q,Wang Z,Chen Z,Wang Z,Hu F,Wang H,Zhou L,Zhang M,Xu J

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells.

Cell reports

Zhao M,Tao F,Venkatraman A,Li Z,Smith SE,Unruh J,Chen S,Ward C,Qian P,Perry JM,Marshall H,Wang J,He XC,Li L

35-5931 was used in Flow cytometry/Cell sorting to report that N-cad+ cells were functional bone and marrow stromal progenitor cells, giving rise to osteoblasts, adipocytes, and chondrocytes both in vitro and in vivo.

Tue Jan 15 00:00:00 EST 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Investigation of tumor-tumor interactions in a double human cervical carcinoma xenograft model in nude mice.

Oncotarget

Mertens B,Cristina de Araujo Nogueira T,Topalis D,Stranska R,Snoeck R,Andrei G

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Apr 24 00:00:00 EDT 2018

Antiviral effects of ferric ammonium citrate.

Cell discovery

Wang H,Li Z,Niu J,Xu Y,Ma L,Lu A,Wang X,Qian Z,Huang Z,Jin X,Leng Q,Wang J,Zhong J,Sun B,Meng G

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Induction of neutrophil apoptosis by a Bcl-2 inhibitor reduces particulate matter-induced lung inflammation.

Aging

Geng X,Wang X,Luo M,Xing M,Wu Y,Li W,Chen Z,Shen H,Ying S

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Jun 26 00:00:00 EDT 2018

Microbiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice.

mSphere

Quach D,Collins F,Parameswaran N,McCabe L,Britton RA

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

35-5931 was used in Flow cytometry/Cell sorting to determine the effect of CCR4 antagonism on human renal cell carcinoma.

Wed Mar 01 00:00:00 EST 2017

Brd4 modulates the innate immune response through Mnk2-eIF4E pathway-dependent translational control of IκBα.

Proceedings of the National Academy of Sciences of the United States of America

Bao Y,Wu X,Chen J,Hu X,Zeng F,Cheng J,Jin H,Lin X,Chen LF

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue May 16 00:00:00 EDT 2017

IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection.

PLoS pathogens

Valanparambil RM,Tam M,Gros PP,Auger JP,Segura M,Gros P,Jardim A,Geary TG,Ozato K,Stevenson MM

35-5931 was used in Flow cytometry/Cell sorting to study the role of interferon regulatory factor-8 on Th2 immunity to Helimosomoides polygyrus bakeri infection.

Sun Oct 01 00:00:00 EDT 2017

Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation.

International journal of molecular medicine

Zhang B,Wang B,Cao S,Wang Y,Wu D

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

MiR-125a Is a critical modulator for neutrophil development.

PLoS genetics

Qin Y,Wu L,Ouyang Y,Zhou P,Zhou H,Wang Y,Ma J,Zhang J,Chen Y,Qian J,Tang Y,Shen N

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Dec 12 00:00:00 EST 2017

BOT-4-one attenuates NLRP3 inflammasome activation: NLRP3 alkylation leading to the regulation of its ATPase activity and ubiquitination.

Scientific reports

Shim DW,Shin WY,Yu SH,Kim BH,Ye SK,Koppula S,Won HS,Kang TB,Lee KH

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Nov 08 00:00:00 EST 2017

Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates.

Nature biotechnology

Vegas AJ,Veiseh O,Doloff JC,Ma M,Tam HH,Bratlie K,Li J,Bader AR,Langan E,Olejnik K,Fenton P,Kang JW,Hollister-Locke J,Bochenek MA,Chiu A,Siebert S,Tang K,Jhunjhunwala S,Aresta-Dasilva S,Dholakia N,Thakrar R,Vietti T,Chen M,Cohen J,Siniakowicz K,Qi M,McGarrigle J,Graham AC,Lyle S,Harlan DM,Greiner DL,Oberholzer J,Weir GC,Langer R,Anderson DG

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Multiple myeloma exosomes establish a favourable bone marrow microenvironment with enhanced angiogenesis and immunosuppression.

The Journal of pathology

Wang J,De Veirman K,Faict S,Frassanito MA,Ribatti D,Vacca A,Menu E

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth.

Stem cells translational medicine

Zheng H,Zou W,Shen J,Xu L,Wang S,Fu YX,Fan W

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

The miR-17∼92 cluster contributes to MLL leukemia through the repression of MEIS1 competitor PKNOX1.

Leukemia research

Mian YA,Zeleznik-Le NJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury.

Cell death & disease

Di Siena S,Gimmelli R,Nori SL,Barbagallo F,Campolo F,Dolci S,Rossi P,Venneri MA,Giannetta E,Gianfrilli D,Feigenbaum L,Lenzi A,Naro F,Cianflone E,Mancuso T,Torella D,Isidori AM,Pellegrini M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Jul 28 00:00:00 EDT 2016

Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice.

BMC genomics

Leist SR,Pilzner C,van den Brand JM,Dengler L,Geffers R,Kuiken T,Balling R,Kollmus H,Schughart K

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sat Feb 27 00:00:00 EST 2016

ILC3 GM-CSF production and mobilisation orchestrate acute intestinal inflammation.

eLife

Pearson C,Thornton EE,McKenzie B,Schaupp AL,Huskens N,Griseri T,West N,Tung S,Seddon BP,Uhlig HH,Powrie F

35-5931 was used in Flow cytometry/Cell sorting to investigate how a small population of innate lymphoid cells (ILCs) has large effects on immune homeostasis, showing that ILC3s produce GM-CSF to orchestrate acute intestinal inflammation.

Mon Jan 18 00:00:00 EST 2016

Functional-genetic dissection of HDAC dependencies in mouse lymphoid and myeloid malignancies.

Blood

Matthews GM,Mehdipour P,Cluse LA,Falkenberg KJ,Wang E,Roth M,Santoro F,Vidacs E,Stanley K,House CM,Rusche JR,Vakoc CR,Zuber J,Minucci S,Johnstone RW

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2015

Apoptotic cells trigger a membrane-initiated pathway to increase ABCA1.

The Journal of clinical investigation

Fond AM,Lee CS,Schulman IG,Kiss RS,Ravichandran KS

35-5931 was used in Flow cytometry/Cell sorting to identify a membrane-initiated pathway that is triggered by apoptotic cells to enhance ABCA1 within engulfing phagocytes.

Wed Jul 01 00:00:00 EDT 2015

Endothelium and NOTCH specify and amplify aorta-gonad-mesonephros-derived hematopoietic stem cells.

The Journal of clinical investigation

Hadland BK,Varnum-Finney B,Poulos MG,Moon RT,Butler JM,Rafii S,Bernstein ID

35-5931 was used in Flow cytometry/Cell sorting to investigate the critical niche components and resident signals required for hematopoietic stem cell induction and self-renewal ex vivo.

Fri May 01 00:00:00 EDT 2015

A far-red fluorescent probe for flow cytometry and image-based functional studies of xenobiotic sequestering macrophages.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Keswani RK,Yoon GS,Sud S,Stringer KA,Rosania GR

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Yersinia pestis targets neutrophils via complement receptor 3.

Cellular microbiology

Merritt PM,Nero T,Bohman L,Felek S,Krukonis ES,Marketon MM

35-5931 was used in Flow cytometry/Cell sorting to identify the factors involved in the targeting of murine splenocytes by Yersinia pestis.

Fri May 01 00:00:00 EDT 2015

Local delivery of VEGF and SDF enhances endothelial progenitor cell recruitment and resultant recovery from ischemia.

Tissue engineering. Part A

Anderson EM,Kwee BJ,Lewin SA,Raimondo T,Mehta M,Mooney DJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

The bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells.

Oncotarget

Wang J,De Veirman K,De Beule N,Maes K,De Bruyne E,Van Valckenborgh E,Vanderkerken K,Menu E

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2015

Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1.

PloS one

Szulzewsky F,Pelz A,Feng X,Synowitz M,Markovic D,Langmann T,Holtman IR,Wang X,Eggen BJ,Boddeke HW,Hambardzumyan D,Wolf SA,Kettenmann H

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2016

Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts.

Stem cell reports

Neri T,Muggeo S,Paulis M,Caldana ME,Crisafulli L,Strina D,Focarelli ML,Faggioli F,Recordati C,Scaramuzza S,Scanziani E,Mantero S,Buracchi C,Sobacchi C,Lombardo A,Naldini L,Vezzoni P,Villa A,Ficara F

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Oct 13 00:00:00 EDT 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Metronomic cyclophosphamide eradicates large implanted GL261 gliomas by activating antitumor Cd8⁺ T-cell responses and immune memory.

Oncoimmunology

Wu J,Waxman DJ

35-5931 was used in Flow cytometry/Cell sorting to prove that single-agent chemotherapy can eradicate large, established tumours and induce long-term immune memory.

Wed Apr 01 00:00:00 EDT 2015

Host adaptation of a bacterial toxin from the human pathogen Salmonella Typhi.

Cell

Deng L,Song J,Gao X,Wang J,Yu H,Chen X,Varki N,Naito-Matsui Y,Galán JE,Varki A

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Dec 04 00:00:00 EST 2014

Macrophages contribute to the cyclic activation of adult hair follicle stem cells.

PLoS biology

Castellana D,Paus R,Perez-Moreno M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Anti-tumor innate immunity activated by intermittent metronomic cyclophosphamide treatment of 9L brain tumor xenografts is preserved by anti-angiogenic drugs that spare VEGF receptor 2.

Molecular cancer

Doloff JC,Chen CS,Waxman DJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Jun 26 00:00:00 EDT 2014

Activation of NLRP3 inflammasome by crystalline structures via cell surface contact.

Scientific reports

Hari A,Zhang Y,Tu Z,Detampel P,Stenner M,Ganguly A,Shi Y

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Dec 02 00:00:00 EST 2014

The cardioprotective protein apolipoprotein A1 promotes potent anti-tumorigenic effects.

The Journal of biological chemistry

Zamanian-Daryoush M,Lindner D,Tallant TC,Wang Z,Buffa J,Klipfell E,Parker Y,Hatala D,Parsons-Wingerter P,Rayman P,Yusufishaq MSS,Fisher EA,Smith JD,Finke J,DiDonato JA,Hazen SL

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Jul 19 00:00:00 EDT 2013

Differential gene expression in mice infected with distinct Toxoplasma strains.

Infection and immunity

Hill RD,Gouffon JS,Saxton AM,Su C

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

A model of GAG/MIP-2/CXCR2 interfaces and its functional effects.

Biochemistry

Rajasekaran D,Keeler C,Syed MA,Jones MC,Harrison JK,Wu D,Bhandari V,Hodsdon ME,Lolis EJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Tue Jul 17 00:00:00 EDT 2012

Virotherapy of canine tumors with oncolytic vaccinia virus GLV-1h109 expressing an anti-VEGF single-chain antibody.

PloS one

Patil SS,Gentschev I,Adelfinger M,Donat U,Hess M,Weibel S,Nolte I,Frentzen A,Szalay AA

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Thu Mar 28 00:00:00 EDT 2013

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Genetic deletion of chemokine receptor Ccr6 decreases atherogenesis in ApoE-deficient mice.

Circulation research

Wan W,Lim JK,Lionakis MS,Rivollier A,McDermott DH,Kelsall BL,Farber JM,Murphy PM

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

Local mesenchymal stem/progenitor cells are a preferential target for initiation of adult soft tissue sarcomas associated with p53 and Rb deficiency.

The American journal of pathology

Choi J,Curtis SJ,Roy DM,Flesken-Nikitin A,Nikitin AY

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2010

The presence of professional phagocytes dictates the number of host cells targeted for Yop translocation during infection.

Cellular microbiology

Durand EA,Maldonado-Arocho FJ,Castillo C,Walsh RL,Mecsas J

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2010

Cellular expression and antimicrobial function of a phylogenetically conserved novel histone 1x-like protein on mouse cells: a potential new class of pattern recognition receptor.

Journal of leukocyte biology

Evans DL,Connor MA,Moss LD,Lackay S,Leary JH,Krunkosky T,Jaso-Friedmann L

35-5931 was used in Flow cytometry/Cell sorting to identify and investigate the function of a H1x-like protein.

Wed Jul 01 00:00:00 EDT 2009

TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis.

Cellular immunology

Drage MG,Pecora ND,Hise AG,Febbraio M,Silverstein RL,Golenbock DT,Boom WH,Harding CV

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Fri Jun 12 00:00:00 EDT 2009

Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling.

Cancer research

Huang B,Zhao J,Shen S,Li H,He KL,Shen GX,Mayer L,Unkeless J,Li D,Yuan Y,Zhang GM,Xiong H,Feng ZH

Tue May 01 00:00:00 EDT 2007

The mouse mammary carcinoma 4T1: characterization of the cellular landscape of primary tumours and metastatic tumour foci.

International journal of experimental pathology

DuPré SA,Redelman D,Hunter KW

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 35-5931-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2007

Hemorrhagic shock-activated neutrophils augment TLR4 signaling-induced TLR2 upregulation in alveolar macrophages: role in hemorrhage-primed lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Fan J,Li Y,Vodovotz Y,Billiar TR,Wilson MA

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Granulocyte Marker; Ly-6C1; Ly-6C2; Ly-6G; Ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C precursor (Ly-6C); Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN

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Gene Aliases: AA682074; AA959465; Gr-1; Gr1; Ly-6C; Ly-6C.2; Ly-6C1; Ly-6C2; Ly-6G; Ly6c; Ly6c1; Ly6c2; Ly6g

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UniProt ID: (Mouse) P0CW02, (Mouse) P0CW03, (Mouse) P35461

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Entrez Gene ID: (Mouse) 17067, (Mouse) 100041546, (Mouse) 546644

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Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine5.5, eBioscience™

Ly-6G/Ly-6C Antibody (35-5931-82) in Flow

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35-5931-82

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