Invitrogen

Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PerCP-eFluor™ 710, eBioscience™

Ly-6G/Ly-6C Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

Ly-6G/Ly-6C Antibody (46-5931-82) in Flow

C57BL/6 mouse bone marrow cells were stained with CD11b Monoclonal Antibody, eFluor 450 (Product # 48-0112-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4031-82) (left) or 0.125 µg of Ly-6G/Ly-6C Monoclonal Antibody, PerCP-eFluor 710 (right). Cells in the myeloid gate were used for analysis.

Ly-6G/Ly-6C Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

46-5931-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Flow Cytometry (Flow)

0.25 µg/test

View 79 publications 79 publications

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RB6-8C5

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848343

Product Specific Information

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (Product # 9668) be used when looking at Ly-6G specific targets.

Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Target Information

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Proceedings of the National Academy of Sciences of the United States of America

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International journal of molecular medicine

Zhang B,Wang B,Cao S,Wang Y,Wu D

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Oncotarget

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Tue Dec 12 00:00:00 EST 2017

BOT-4-one attenuates NLRP3 inflammasome activation: NLRP3 alkylation leading to the regulation of its ATPase activity and ubiquitination.

Scientific reports

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Combinatorial hydrogel library enables identification of materials that mitigate the foreign body response in primates.

Nature biotechnology

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Stem cells translational medicine

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Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

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Sat Oct 01 00:00:00 EDT 2016

Effects of ketanserin on experimental colitis in mice and macrophage function.

International journal of molecular medicine

Xiao J,Shao L,Shen J,Jiang W,Feng Y,Zheng P,Liu F

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Tue Mar 01 00:00:00 EST 2016

The miR-17∼92 cluster contributes to MLL leukemia through the repression of MEIS1 competitor PKNOX1.

Leukemia research

Mian YA,Zeleznik-Le NJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Activated c-Kit receptor in the heart promotes cardiac repair and regeneration after injury.

Cell death & disease

Di Siena S,Gimmelli R,Nori SL,Barbagallo F,Campolo F,Dolci S,Rossi P,Venneri MA,Giannetta E,Gianfrilli D,Feigenbaum L,Lenzi A,Naro F,Cianflone E,Mancuso T,Torella D,Isidori AM,Pellegrini M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Jul 28 00:00:00 EDT 2016

Influenza H3N2 infection of the collaborative cross founder strains reveals highly divergent host responses and identifies a unique phenotype in CAST/EiJ mice.

BMC genomics

Leist SR,Pilzner C,van den Brand JM,Dengler L,Geffers R,Kuiken T,Balling R,Kollmus H,Schughart K

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Sat Feb 27 00:00:00 EST 2016

Functional-genetic dissection of HDAC dependencies in mouse lymphoid and myeloid malignancies.

Blood

Matthews GM,Mehdipour P,Cluse LA,Falkenberg KJ,Wang E,Roth M,Santoro F,Vidacs E,Stanley K,House CM,Rusche JR,Vakoc CR,Zuber J,Minucci S,Johnstone RW

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2015

A far-red fluorescent probe for flow cytometry and image-based functional studies of xenobiotic sequestering macrophages.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Keswani RK,Yoon GS,Sud S,Stringer KA,Rosania GR

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Local delivery of VEGF and SDF enhances endothelial progenitor cell recruitment and resultant recovery from ischemia.

Tissue engineering. Part A

Anderson EM,Kwee BJ,Lewin SA,Raimondo T,Mehta M,Mooney DJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

The bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells.

Oncotarget

Wang J,De Veirman K,De Beule N,Maes K,De Bruyne E,Van Valckenborgh E,Vanderkerken K,Menu E

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2015

Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1.

PloS one

Szulzewsky F,Pelz A,Feng X,Synowitz M,Markovic D,Langmann T,Holtman IR,Wang X,Eggen BJ,Boddeke HW,Hambardzumyan D,Wolf SA,Kettenmann H

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2016

Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts.

Stem cell reports

Neri T,Muggeo S,Paulis M,Caldana ME,Crisafulli L,Strina D,Focarelli ML,Faggioli F,Recordati C,Scaramuzza S,Scanziani E,Mantero S,Buracchi C,Sobacchi C,Lombardo A,Naldini L,Vezzoni P,Villa A,Ficara F

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Oct 13 00:00:00 EDT 2015

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Host adaptation of a bacterial toxin from the human pathogen Salmonella Typhi.

Cell

Deng L,Song J,Gao X,Wang J,Yu H,Chen X,Varki N,Naito-Matsui Y,Galán JE,Varki A

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Dec 04 00:00:00 EST 2014

Macrophages contribute to the cyclic activation of adult hair follicle stem cells.

PLoS biology

Castellana D,Paus R,Perez-Moreno M

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Mon Dec 01 00:00:00 EST 2014

Anti-tumor innate immunity activated by intermittent metronomic cyclophosphamide treatment of 9L brain tumor xenografts is preserved by anti-angiogenic drugs that spare VEGF receptor 2.

Molecular cancer

Doloff JC,Chen CS,Waxman DJ

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Jun 26 00:00:00 EDT 2014

Activation of NLRP3 inflammasome by crystalline structures via cell surface contact.

Scientific reports

Hari A,Zhang Y,Tu Z,Detampel P,Stenner M,Ganguly A,Shi Y

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Dec 02 00:00:00 EST 2014

Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros.

Nature immunology

Schjerven H,McLaughlin J,Arenzana TL,Frietze S,Cheng D,Wadsworth SE,Lawson GW,Bensinger SJ,Farnham PJ,Witte ON,Smale ST

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio)

Grosselin J,Sii-Felice K,Payen E,Chretien S,Tronik-Le Roux D,Leboulch P

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

The cardioprotective protein apolipoprotein A1 promotes potent anti-tumorigenic effects.

The Journal of biological chemistry

Zamanian-Daryoush M,Lindner D,Tallant TC,Wang Z,Buffa J,Klipfell E,Parker Y,Hatala D,Parsons-Wingerter P,Rayman P,Yusufishaq MSS,Fisher EA,Smith JD,Finke J,DiDonato JA,Hazen SL

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Fri Jul 19 00:00:00 EDT 2013

Differential gene expression in mice infected with distinct Toxoplasma strains.

Infection and immunity

Hill RD,Gouffon JS,Saxton AM,Su C

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Virotherapy of canine tumors with oncolytic vaccinia virus GLV-1h109 expressing an anti-VEGF single-chain antibody.

PloS one

Patil SS,Gentschev I,Adelfinger M,Donat U,Hess M,Weibel S,Nolte I,Frentzen A,Szalay AA

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Mar 28 00:00:00 EDT 2013

Hepatocyte-specific S100a8 and S100a9 transgene expression in mice causes Cxcl1 induction and systemic neutrophil enrichment.

Cell communication and signaling : CCS

Wiechert L,Németh J,Pusterla T,Bauer C,De Ponti A,Manthey S,Marhenke S,Vogel A,Klingmüller U,Hess J,Angel P

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Sat Dec 15 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Genetic deletion of chemokine receptor Ccr6 decreases atherogenesis in ApoE-deficient mice.

Circulation research

Wan W,Lim JK,Lionakis MS,Rivollier A,McDermott DH,Kelsall BL,Farber JM,Murphy PM

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

A tritherapy combination of a fusion protein vaccine with immune-modulating doses of sequential chemotherapies in an optimized regimen completely eradicates large tumors in mice.

International journal of cancer

Song X,Guo W,Cui J,Qian X,Yi L,Chang M,Cai Q,Zhao Q

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2011

DNA alkylating therapy induces tumor regression through an HMGB1-mediated activation of innate immunity.

Journal of immunology (Baltimore, Md. : 1950)

Guerriero JL,Ditsworth D,Catanzaro JM,Sabino G,Furie MB,Kew RR,Crawford HC,Zong WX

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2011

Brain dendritic cells in ischemic stroke: time course, activation state, and origin.

Brain, behavior, and immunity

Felger JC,Abe T,Kaunzner UW,Gottfried-Blackmore A,Gal-Toth J,McEwen BS,Iadecola C,Bulloch K

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2010

Local mesenchymal stem/progenitor cells are a preferential target for initiation of adult soft tissue sarcomas associated with p53 and Rb deficiency.

The American journal of pathology

Choi J,Curtis SJ,Roy DM,Flesken-Nikitin A,Nikitin AY

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2010

The presence of professional phagocytes dictates the number of host cells targeted for Yop translocation during infection.

Cellular microbiology

Durand EA,Maldonado-Arocho FJ,Castillo C,Walsh RL,Mecsas J

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2010

Combination of all-trans retinoic acid and a human papillomavirus therapeutic vaccine suppresses the number and function of immature myeloid cells and enhances antitumor immunity.

Cancer science

Song X,Ye D,Liu B,Cui J,Zhao X,Yi L,Liang J,Song J,Zhang Z,Zhao Q

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2009

Cellular expression and antimicrobial function of a phylogenetically conserved novel histone 1x-like protein on mouse cells: a potential new class of pattern recognition receptor.

Journal of leukocyte biology

Evans DL,Connor MA,Moss LD,Lackay S,Leary JH,Krunkosky T,Jaso-Friedmann L

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2009

TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis.

Cellular immunology

Drage MG,Pecora ND,Hise AG,Febbraio M,Silverstein RL,Golenbock DT,Boom WH,Harding CV

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Fri Jun 12 00:00:00 EDT 2009

The mouse mammary carcinoma 4T1: characterization of the cellular landscape of primary tumours and metastatic tumour foci.

International journal of experimental pathology

DuPré SA,Redelman D,Hunter KW

Published figure using Ly-6G/Ly-6C monoclonal antibody (Product # 46-5931-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Granulocyte Marker; Ly-6C1; Ly-6C2; Ly-6G; Ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C precursor (Ly-6C); Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN

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Gene Aliases: AA682074; AA959465; Gr-1; Gr1; Ly-6C; Ly-6C.2; Ly-6C1; Ly-6C2; Ly-6G; Ly6c; Ly6c1; Ly6c2; Ly6g

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UniProt ID: (Mouse) P0CW02, (Mouse) P0CW03, (Mouse) P35461

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Entrez Gene ID: (Mouse) 17067, (Mouse) 100041546, (Mouse) 546644

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Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PerCP-eFluor™ 710, eBioscience™

Ly-6G/Ly-6C Antibody (46-5931-82) in Flow

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46-5931-82

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