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Ly-6G/Ly-6C Antibody (M016T02R03) in Flow
C57BL/6 mouse splenocytes were unstained (left) or stained with 0.05 µg of Ly-6G/Ly-6C Monoclonal Antibody, NovaFluor Red 700 (Product # m016t02r03) (right). All cells were co-stained with CD45R (B220) (RA3-6B2) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
M016T02R03
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
RB6-8C5
Conjugate
Excitation/Emission Max
637/701 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2910816
Product Specific Information
Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.
eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (Product # 9668) be used when looking at Ly-6G specific targets.
Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.05 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer . Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100µL of cell sample containing 10^3 to 10^8 cells.
Excitation: 639 nm; Emission: 700 nm; Laser: 633-640 nm (Red) Laser
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more .
Target Information
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Granulocyte Marker; Ly-6C1; Ly-6C2; Ly-6G; Ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C precursor (Ly-6C); Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN
Gene Aliases: AA682074; AA959465; Gr-1; Gr1; Ly-6C; Ly-6C.2; Ly-6C1; Ly-6C2; Ly-6G; Ly6c; Ly6c1; Ly6c2; Ly6g
UniProt ID: (Mouse) P0CW02, (Mouse) P0CW03, (Mouse) P35461
Entrez Gene ID: (Mouse) 17067, (Mouse) 100041546, (Mouse) 546644
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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