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RNA Structure/Function Studies |
Tools for RNA structure/function research
RNA structure is thought to play a central role in many cellular processes, including transcription initiation, elongation and termination, mRNA splicing, and retroviral infection of eukaryotic cells. Elucidating the mechanistic aspects of these intricate processes will require detailed understanding of the underlying RNA structure. RNA molecules usually possess a variety of single-stranded and double-stranded regions that give rise to complex three-dimensional structures. These structures are involved in the molecule's interactions with other nucleic acids, proteins, and small molecules. We have developed an extensive portfolio of products for the synthesis and modification of RNA in order to further understand the role of RNA structure.
Enzymes
- RNA-Grade Ribonucleases: A collection of ribonucleases (RNases A and T1) that are optimized for researchers performing RNA structure, RNA sequencing, protein footprinting and boundary experiments.
- Poly(A)Polymerase: Catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion.
- T7 RNA Polymerase: Catalyzes the 5´→3´ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
- SP6 RNA Polymerase: Catalyzes the 5´→3´ synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter and incorporates modified nucleotides.
- Anza T4 Polynucleotide Kinase: Transfers the terminal phosphate of ATP to the 5´ hydroxyl terminus of DNA or RNA. Used for 5' end labeling of oligonucleotides and polynucleotides.
- T4 RNA Ligase: Catalyzes the formation of a phosphodiester linkage between a 5´-phosphoryl-terminated ribonucleic acid and a 3´-hydroxyl-terminated ribonucleic acid.
- KinaseMax™ 5' End Labeling Kit: For end-labeling DNA, RNA, and oligonucleotides using T4 polynucleotide kinase and [gamma-32P]ATP.
- Terminal Transferase: Catalyzes the addition of deoxynucleotides to the 3´ hydroxyl terminus of DNA.
- CIP (calf Intestinal Phosphatase): Phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.
- BAP (Bacterial Alkaline Phosphatase): Removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction
In Vitro transcription kits
- MEGAshortscript™ High Yield Transcription Kit: Efficiently transcribes large quantities of RNA from short templates, <300 bases.
Other
- Cap Analog & Variants: m7G(5´)ppp(5´)G (Cap Analog) and Cap Variants. The Cap Analog is used for synthesis of
5´-capped RNA molecules.
- Modified UTPs: Incorporate these nucleotides to confer unique characteristics and test for particular reactive groups.
References
- Moine H, Ehresmann B, Ehresmann C, and Romby P. (1998) Probing RNA structure and function in solution. In: Simons RW and Grunberg-Manago M (editors). RNA Structure and Function, Cold Spring Harbor Laboratory Press. p. 77-115.
- Knapp G. (1989) Enzymatic approaches to probing of RNA secondary and tertiary structure. Methods Enzymol 180:192-212. Abstract
- Krol A and Carbon P. (1989) A guide for probing native small nuclear RNA and ribonucleoprotein structures. Methods Enzymol 180:212-227. Abstract
- Tinoco I Jr, and Bustamante C. (1999) How RNA folds. J Mol Biol 293(2):271-281. Abstract
- Doudna J A. (2000) Structural genomics of RNA. Nature Struct Biol 7 Suppl:954-956. Abstract
TechNotes Articles
Protocols
Stylesheet for Classic Wide Template adjustments
仅供科研使用，不可用于诊断目的。