Transcriptome Analysis

Whole-transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression. We offer an extensive range of products, from microarray labeling reagents to next-generation sequencing reagents and instrumentation, to help capture the complexity of whole-transcriptome analysis for your research.

Successful whole transcriptome analysis depends on RNA quality and efficient conversion to cDNA libraries. Invitrogen and Ambion Transcriptome analysis sample preparation solutions are designed to be optimized for your desired workflow. Optimized sample preparation means complete solutions from one source, that minimize further manipulation and are flexible enough to work with a broad range of starting samples, for greater confidence, and accountability to help troubleshoot when needed.

• Learn more about Transcriptome mRNA Purification
• Learn more about Transcriptome RNA Enrichment (rRNA Depletion)
• View the Transcriptome RNA Purification Selection Guide

Gene arrays have become a powerful approach for comparing complex sample RNA populations. Using array analysis, the expression profiles of normal and tumor tissues, treated and untreated cell cultures, developmental stages of an organism or tissue, and different tissues can be compared to gain a better understanding of the Transcriptome.

• Learn more about Expression Array Labeling Kits

With a dynamic range to detect subtle changes in expression level in a hypothesis-neutral environment, next-generation sequencing helps provide an understanding of biological response to stimuli or environmental changes. The Ion Proton System offers a reliable sequencing workflow for your research that combines simple sample preparation and intuitive data analysis with the flexibility to perform whole-transcriptome sequencing for identification and quantification of novel and known transcripts, or targeted transcriptome sequencing for simple, gene-level expression analysis.

• Learn more about transcriptome sequencing on the Ion Proton System