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ADME/Tox Studies and Products
We strive to offer industry-leading ADME/Tox products that provide physiologically relevant results, so you can make the right decisions to support your research. We are a team of hepatic scientists helping fellow scientists-supporting with our products and services, and participating in the advancement of research in 3D, enzyme induction, CYP inhibition, hepatic transport, and other metabolism-related (DMPK) fields and applications. We offer a comprehensive portfolio of tools to support your workflow across every step.
Overview of the cell lines, culture conditions, and analysis techniques that are being used in the transition from 2D to 3D cell models in the 3D Cell Culture & Analysis Handbook.
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Have fun with mazes, lab awards, a word search, and more in the 3D Cell Culture & Analysis Activity Booklet.
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See how our R&D team is transfecting cryopreserved human and fresh rat hepatocytes
We offer high-grade hepatic systems (cells, media, supplements and matrices), subcellular fractions, and transport systems backed by extensive expertise in hepatic systems and decades of hepatocyte culture experience
- Extensive expertise with decades of hepatocyte culture experience
- Dedicated technical and field support to help with your ADME/DMPK research
- A broad selection of cells, media, and product guidance necessary to create physiologically relevant co-culture models
- Broad selection of human and animal hepatic cells to support your ADME/DMPK research
Transition seamlessly through all your ADME needs.
Hepatic cells
Our extensive selection of premier hepatocytes and culture products is backed by decades of experience. Human and animal hepatocyte cells are isolated in-house by our specially trained scientists, helping to ensure the highest quality standards translate into your ability to draw in-vitro/in-vivo correlations. Each of our lots is tested for monolayer formation, attachment efficiency, proper morphology, viability, and phase I and II enzyme activities. Our human hepatocytes are pre-qualified for a variety of applications including metabolism, induction, and transporter studies.
We can help you seamlessly transition through your workflow with a complete cell culture system of Gibco 2D and 3D hepatocytes, media, and supplements. From suspension to adherent cultures, we offer thawing and recovery medium to improve hepatic cell viability, extracellular matrixes for a more in situ environment offering more physiological relevant results, and various media and supplements to help maintain the health of your hepatic cells.
Our most used products:
- Single & Pooled Donor Human Hepatocytes
- Spheroid-Qualified Hepatocytes
- HepaRG
- Human stellates
- Human and rat kupffers
- Animal hepatocytes
Our specially trained isolation experts bring your scientists premier hepatocytes and help enable your success.
Media, supplements, & matrices
Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to ease centrifugation, or are ready to set up your long-term culture with hepatocyte plating supplements and William’s E Medium we offer premier Gibco media to support all stages of hepatocyte cell culture.
The breadth of our Gibco media and reagents offering is designed to provide an ideal environment for adherent and suspension hepatocytes
- Supplements and media for adherent and suspension culture types
- Media for every step in your cell culture workflow
- Customizable medium—begin your custom journey here
| Step 1. Thawing | Step 2. Washing | Step 3. Plating | Step 4. Feeding | Step 5. Extending culture time |
|---|---|---|---|---|
 |  |  |  |  |
| Cryopreserved Hepatocyte Recovery Medium is a ready-to-use medium that is specifically designed to improve cell viability and overall quality. Simply warm up the 50 ml tube and add thawed hepatocytes. | Hepatocyte Wash Medium is a ready-to-use medium to ###. It is specially formulation to help of cell membrane and maintain viability during centrifugation. | Primary Hepatocyte Thawing and Plating Supplement is designed specifically for the initial plating of fresh or cryopreserved hepatocytes. | Primary Hepatocyte Maintenance Supplements is specifically optimized for your long-term cell culture feeding needs. | HepExtend can be used to extend your culture >10 days while maintaining normal cell morphology and bile canaliculi. |
| *Williams E Medium can be used for short and long-term cell culture of hepatocyte cells from human and animal species, as well as HepaRG cells. | ||||
Extracellular matrices
Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, to collagen (bulk or pre-plated in 6-, 24-, or 96-well formats), and AlgiMatrix (an animal-free bioscaffold) for creating higher-fidelity hepatic models.
Subcellular fractions & transporter products
Subcellular fractions
Our subcellular fractions, derived from the endoplasmic reticulum of hepatocytes, contain an assortment of CYP450 enzymes for assessing the in vitro metabolism of drug candidates. They are suitable for a wide range of applications including: cytochrome P450 inhibition studies, metabolic stability studies, cytochrome P450 phenotyping, and metabolite characterization.
- Subcellular fractions from a variety of tox species including human, cynomolgus monkey, dog, rat, and mouse
- Large, pooled lots for reproducible, long-term studies
- Microsomes—for studies needing Phase I CYP enzymes, suitable for drug metabolism and drug interaction studies
- S9—for studies needing Phase I & Phase II CYP enzymes, suitable for drug metabolism and drug interaction studies
- Cytosol—for studies needing Phase II CYP enzymes, suitable for metabolic profiling
Transporter products
We offer an extensive selection of GenoMembrane ATP-binding cassette (ABC) transporter vesicles and GenoMembrane TRANSiPORT human solute carrier transporter cells to help you evaluate your compound’s transporter interactions and accelerate your answers.
GenoMembrane ATP-binding Cassette (ABC) Transporter Vesicles
- Identify drug transporter interactions and if your drug is a substrate or inhibitor of a specific efflux transporter
- A broad selection of transporters from multiple species including human, dog, monkey, and rat
- The “inside-out” design of the vesicles allow for the import of substrate directly into the vesicles for quantitative measurements
GenoMembrane TRANSiPORT Human SLC Transporter Cells
- Assess potential for transporter-mediated drug metabolism
- Easy-to-use format
- Obtain high-quality results with a large signal-to-noise ratio
- Easy-to-use product enables data generation in 2 days
Assays instrumentation
From determining hepatocyte cell viability, conducting new compound affinity testing, to analyzing ADME-related gene expression, we offer assays, reagents, and instrumentation to support all ADME applications.
Assays
Nuclear receptors– Nuclear receptors are a superfamily of transcription factors that can mediate transcription regulation in ADME-relevant genes. To facilitate your drug discovery efforts through the study of nuclear receptors, we have developed highly sensitive Invitrogen LanthaScreen Nuclear Receptor Competitive Binding Assays, suitable for high-throughput screening.
TaqMan® Assays– Applied Biosystems TaqMan® Marker and Reporter assays are optimized for detection and quantitation of DNA or RNA sequences encoding common marker and reporter proteins and can be useful for determining successful transfection of your fluorescent fusion protein. TaqMan® Drug Metabolism Genotyping Assays are designed to detect polymorphisms in high-value drug metabolizing enzyme pharmacogenetics markers, including PharmaADME core markers. Our assays, pre-plated into Fast or standard 96- or 384-well plates, detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters.
Instruments
EVOS FL Auto Cell Imaging System– Our EVOS microscopes are designed for biologists, easy-to-use, versatile microscopes that reinvent your hepatic cell imaging routines to produce publication quality images and data in no time. Our EVOS systems offer top-of-the-line imaging components including high-quality cameras and optics, LED illumination, and easy-to-use image capture and processing software.
Plasma Protein Binding Assays
The RED (Rapid Equilibrium Dialysis) and Competition RED systems utilize a disposable device format that is automation-friendly, providing convenience for scientists conducting plasma or tissue-to-plasma protein-binding studies. The inserts are ready to use and do not require any pre-conditioning to the membrane. The single-use format for the RED Device is especially convenient for labs using radioactive materials because the plate can be disposed of easily to help avoid contamination and cleaning. These devices have been tested in assays with high-, medium- and low-protein binding compounds.
- Disposable tubes—require no presoaking, assembly or specialized equipment
- Short incubation time—the high membrane surface-to-volume ratio enables equilibrium to be reached in as few as 100 minutes
- Flexible—can be used for any number of assays (1–48 assays/plate) and the 96-well footprint is suitable for automated liquid handlers
- Robust—compartmentalized design helps minimize potential for cross contamination or leakage
- Validated—each lot is functionally tested in a protein-binding assay to confirm performance
Hepatic cells
Our extensive selection of premier hepatocytes and culture products is backed by decades of experience. Human and animal hepatocyte cells are isolated in-house by our specially trained scientists, helping to ensure the highest quality standards translate into your ability to draw in-vitro/in-vivo correlations. Each of our lots is tested for monolayer formation, attachment efficiency, proper morphology, viability, and phase I and II enzyme activities. Our human hepatocytes are pre-qualified for a variety of applications including metabolism, induction, and transporter studies.
We can help you seamlessly transition through your workflow with a complete cell culture system of Gibco 2D and 3D hepatocytes, media, and supplements. From suspension to adherent cultures, we offer thawing and recovery medium to improve hepatic cell viability, extracellular matrixes for a more in situ environment offering more physiological relevant results, and various media and supplements to help maintain the health of your hepatic cells.
Our most used products:
- Single & Pooled Donor Human Hepatocytes
- Spheroid-Qualified Hepatocytes
- HepaRG
- Human stellates
- Human and rat kupffers
- Animal hepatocytes
Our specially trained isolation experts bring your scientists premier hepatocytes and help enable your success.
Media, supplements, & matrices
Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to ease centrifugation, or are ready to set up your long-term culture with hepatocyte plating supplements and William’s E Medium we offer premier Gibco media to support all stages of hepatocyte cell culture.
The breadth of our Gibco media and reagents offering is designed to provide an ideal environment for adherent and suspension hepatocytes
- Supplements and media for adherent and suspension culture types
- Media for every step in your cell culture workflow
- Customizable medium—begin your custom journey here
| Step 1. Thawing | Step 2. Washing | Step 3. Plating | Step 4. Feeding | Step 5. Extending culture time |
|---|---|---|---|---|
| | | | |
| Cryopreserved Hepatocyte Recovery Medium is a ready-to-use medium that is specifically designed to improve cell viability and overall quality. Simply warm up the 50 ml tube and add thawed hepatocytes. | Hepatocyte Wash Medium is a ready-to-use medium to ###. It is specially formulation to help of cell membrane and maintain viability during centrifugation. | Primary Hepatocyte Thawing and Plating Supplement is designed specifically for the initial plating of fresh or cryopreserved hepatocytes. | Primary Hepatocyte Maintenance Supplements is specifically optimized for your long-term cell culture feeding needs. | HepExtend can be used to extend your culture >10 days while maintaining normal cell morphology and bile canaliculi. |
| *Williams E Medium can be used for short and long-term cell culture of hepatocyte cells from human and animal species, as well as HepaRG cells. | ||||
Extracellular matrices
Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, to collagen (bulk or pre-plated in 6-, 24-, or 96-well formats), and AlgiMatrix (an animal-free bioscaffold) for creating higher-fidelity hepatic models.
Subcellular fractions & transporter products
Subcellular fractions
Our subcellular fractions, derived from the endoplasmic reticulum of hepatocytes, contain an assortment of CYP450 enzymes for assessing the in vitro metabolism of drug candidates. They are suitable for a wide range of applications including: cytochrome P450 inhibition studies, metabolic stability studies, cytochrome P450 phenotyping, and metabolite characterization.
- Subcellular fractions from a variety of tox species including human, cynomolgus monkey, dog, rat, and mouse
- Large, pooled lots for reproducible, long-term studies
- Microsomes—for studies needing Phase I CYP enzymes, suitable for drug metabolism and drug interaction studies
- S9—for studies needing Phase I & Phase II CYP enzymes, suitable for drug metabolism and drug interaction studies
- Cytosol—for studies needing Phase II CYP enzymes, suitable for metabolic profiling
Transporter products
We offer an extensive selection of GenoMembrane ATP-binding cassette (ABC) transporter vesicles and GenoMembrane TRANSiPORT human solute carrier transporter cells to help you evaluate your compound’s transporter interactions and accelerate your answers.
GenoMembrane ATP-binding Cassette (ABC) Transporter Vesicles
- Identify drug transporter interactions and if your drug is a substrate or inhibitor of a specific efflux transporter
- A broad selection of transporters from multiple species including human, dog, monkey, and rat
- The “inside-out” design of the vesicles allow for the import of substrate directly into the vesicles for quantitative measurements
GenoMembrane TRANSiPORT Human SLC Transporter Cells
- Assess potential for transporter-mediated drug metabolism
- Easy-to-use format
- Obtain high-quality results with a large signal-to-noise ratio
- Easy-to-use product enables data generation in 2 days
Assays instrumentation
From determining hepatocyte cell viability, conducting new compound affinity testing, to analyzing ADME-related gene expression, we offer assays, reagents, and instrumentation to support all ADME applications.
Assays
Nuclear receptors– Nuclear receptors are a superfamily of transcription factors that can mediate transcription regulation in ADME-relevant genes. To facilitate your drug discovery efforts through the study of nuclear receptors, we have developed highly sensitive Invitrogen LanthaScreen Nuclear Receptor Competitive Binding Assays, suitable for high-throughput screening.
TaqMan® Assays– Applied Biosystems TaqMan® Marker and Reporter assays are optimized for detection and quantitation of DNA or RNA sequences encoding common marker and reporter proteins and can be useful for determining successful transfection of your fluorescent fusion protein. TaqMan® Drug Metabolism Genotyping Assays are designed to detect polymorphisms in high-value drug metabolizing enzyme pharmacogenetics markers, including PharmaADME core markers. Our assays, pre-plated into Fast or standard 96- or 384-well plates, detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters.
Instruments
EVOS FL Auto Cell Imaging System– Our EVOS microscopes are designed for biologists, easy-to-use, versatile microscopes that reinvent your hepatic cell imaging routines to produce publication quality images and data in no time. Our EVOS systems offer top-of-the-line imaging components including high-quality cameras and optics, LED illumination, and easy-to-use image capture and processing software.
Plasma Protein Binding Assays
The RED (Rapid Equilibrium Dialysis) and Competition RED systems utilize a disposable device format that is automation-friendly, providing convenience for scientists conducting plasma or tissue-to-plasma protein-binding studies. The inserts are ready to use and do not require any pre-conditioning to the membrane. The single-use format for the RED Device is especially convenient for labs using radioactive materials because the plate can be disposed of easily to help avoid contamination and cleaning. These devices have been tested in assays with high-, medium- and low-protein binding compounds.
- Disposable tubes—require no presoaking, assembly or specialized equipment
- Short incubation time—the high membrane surface-to-volume ratio enables equilibrium to be reached in as few as 100 minutes
- Flexible—can be used for any number of assays (1–48 assays/plate) and the 96-well footprint is suitable for automated liquid handlers
- Robust—compartmentalized design helps minimize potential for cross contamination or leakage
- Validated—each lot is functionally tested in a protein-binding assay to confirm performance
Buy Gibco Human Spheroid-Qualified Hepatocytes today
Review our most recent posters from ISSX 2018 on 3D applications in Liver Modeling and Hepatic research:
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Sustainable solutions
We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
Featured ADME/Tox Products
The Gibco and Invitrogen brands by Thermo Fisher Scientific provide cell-based products to help accelerate the discovery and development of new therapeutics. Our comprehensive portfolio of hepatocytes, media, and supplements offer the stable foundation you need to confidently screen compounds for potential effects on liver function in both 2D and 3D models.
Review our offering of products to support your ADME/tox and DMPK needs and reach out to our team of hepatic scientists that can help you integrate these powerful tools in addition to providing hands-on training as you continue to make breakthroughs that will change the world of research and develop better therapies.
GIBCO Hepatocytes
Gibco human and animal hepatocytes and cell culture media, supplements & reagents
Transporter Products
GenoMembrane Transporter Vesicles and Transporter-qualified Hepatocytes
Toxicology Products
Assays to identify indicators of both biochemical and cellular toxicity
Nuclear Receptor ADME Assays
Our LanthaScreen PXR (SXR) and CAR Competitive Binding Assays contains all the reagents you need to conduct compound affinity testing
Plasma Protein Binding Equilibrium Dialysis
Rapid Equilibrium Dialysis (RED) device offers an easy, fast and reliable system for performing plasma protein-binding assays, a critical step in drug development.
Resources
Support
Technical support
We offer one-on-one training with the help of our Field Application Scientists (FAS team) from basic start-up questions and demos up-to getting started in 3D for ADME/DMPK research. reach out today to speak to our team of experts.
Drug Discovery & Development Support Center
Find tips, troubleshooting help, and resources for support related to your drug discovery experiments.
3D Learning Center
Access our resources on culture and analysis of 3D models.
3D Support Center
Culture and analyze organoids and spheroids with confidence using validated protocols and educational resources.
For Research Use Only. Not for use in diagnostic procedures.