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Invitrogen
Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), Alexa Fluor™ 561, eBioscience™
FIGURE: 1 / 2
Ly-6G/Ly-6C Antibody (505-5931-82) in Flow
Alexa Fluor 561 non-specific staining of B cells in the SJL strain of mice. Splenocytes from BALB/c (left) and SJL (right) strains of mice were stained with Anti-Mouse CD19 Monoclonal Antibody conjugated to FITC and a non-cross-reactive, human-specific monoclonal antibody conjugated to Alexa Fluor 561. These data show that Alexa Fluor 561-conjugated antibodies non-specifically stain B cells in SJL mice (right) and outbred, Swiss Webster mice (data not shown). Non-specific staining has not been observed in BALB/c mice (left) and C57BL/6 mic... View More
Product Details
505-5931-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RB6-8C5
Conjugate
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896227
Product Specific Information
Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.
eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (Product # 9668) be used when looking at Ly-6G specific targets.
Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.
Our internal testing shows that Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.
Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610.
Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser.
Target Information
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Granulocyte Marker; Ly-6C1; Ly-6C2; Ly-6G; Ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6C precursor (Ly-6C); Lymphocyte antigen 6C1; Lymphocyte antigen 6C2; Lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN
Gene Aliases: AA682074; AA959465; Gr-1; Gr1; Ly-6C; Ly-6C.2; Ly-6C1; Ly-6C2; Ly-6G; Ly6c; Ly6c1; Ly6c2; Ly6g
UniProt ID: (Mouse) P0CW02, (Mouse) P0CW03, (Mouse) P35461
Entrez Gene ID: (Mouse) 17067, (Mouse) 100041546, (Mouse) 546644
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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