Table 4.1 Biotinylation and desthiobiotinylation reagents.

Reactive Moiety Biotin Derivative (Cat. No.) DSB-X Biotin Derivative (Cat. No.)
Aliphatic amineA1593, A1594, B1596 (XX), N6356 
AlkyneB10185 
AzideB10184 
Carboxylic acidB1595, B20656, B1592D20657
DNP-X–biocytin-X, SEB2604, F6348 (F) 
HydrazideB1603, B2600 (XX)D20653 (X)
HydroxylamineA10550 
IodoacetamideB1591D30753
MaleimideM1602 
Succinimidyl ester (SE)B1513, B1582 (X), B1606 (XX), B6353 (SSE)(X), B6352 (SSE)(XX), A10495 (APEX), B30010 (Micro), B30756 (Micro), F2610 (F), F6347 (F)D20655 (D)
TS-Link thiosulfate T30754
(APEX) = APEX Antibody Labeling Kit. (D) = DSB-X Biotin Protein Labeling Kit. (F) = FluoReporter Protein Labeling Kit. (Micro) = Biotin-XX Microscale Protein Labeling Kit. (SE) = Succinimidyl ester. (SSE) = Sulfosuccinimidyl ester. (X) = Aminohexanoyl (7-atom) spacer separating the biotin or desthiobiotin and the reactive moiety. (XX) = Aminohexanoylaminohexanoyl (14-atom) spacer separating the biotin and the reactive moiety.

For Research Use Only. Not for use in diagnostic procedures.