SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9.2 | Thermo Fisher Scientific - CL

Table 9.2 SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains.

Dye (Cat #)	Ex/Em *	Major Applications	Features
SYPRO Ruby protein gel stain (S12000, S12001, S21900)	280, 450/610	2D gels, IEF gels, 1D SDS-PAGE	High sensitivity (1–2 ng/band; comparable to silver staining) Linear quantitation range over three orders of magnitude
SYPRO Orange protein gel stain (S6650, S6651)	300, 470/570	1D SDS-PAGE	Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining) Little protein-to-protein variability Linear quantitation range over three orders of magnitude
SYPRO Red protein gel stain (S6653, S6654)	300, 550/630	1D SDS-PAGE	Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining) Little protein-to-protein variability Linear quantitation range over three orders of magnitude
SYPRO Tangerine protein gel stain (S12010)	300, 490/640	1D SDS-PAGE, blotting applications, zymography, electroelution	Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining) Little protein-to-protein variability Linear quantitation range over three orders of magnitude No fixation required
Coomassie Fluor Orange protein gel stain (C33250, C33251)	300, 470/570	1D SDS-PAGE	Premixed and ready-to-use solution Very good sensitivity (8 ng/band; higher than Coomassie brilliant blue staining) Little protein-to-protein variability Linear quantitation range over at least two orders of magnitude
All SYPRO gel and blot stains are compatible with Edman sequencing and mass spectrometry. * Excitation (Ex) and emission (Em) maxima, in nm. For maximum sensitivity, use excitation sources and optical filters matched to these values.

For Research Use Only. Not for use in diagnostic procedures.