Parallel performance comparison of fluo-3 and fluo-4 on Molecular Devices FLIPR system—Table 19.3

Indicator	µM *	Time *(min)	Fluorescence †		Increase §
Indicator	µM *	Time *(min)	Basal	Stimulated ‡	Increase §
Fluo-3	4	60	1700	5700	3.4-fold
Fluo-4	4	60	4900	21300	4.3-fold
Fluo-4	2	30	1200	5400	4.5-fold
* CHO cells stably transfected with rat muscarinic M₁ receptors were loaded with fluo-3 AM or fluo-4 AM according to standard protocols, with variations in indicator concentration (µM) and incubation time, as shown. † Relative fluorescence intensities after subtraction of background fluorescence. ‡ Cells were stimulated by addition of the muscarinic agonist carbachol (50 µM). § Ratio of stimulated to basal fluorescence intensities.

For Research Use Only. Not for use in diagnostic procedures.

Parallel Performance Comparison of Fluo-3 and Fluo-4 on Molecular Devices FLIPR System—Table 19.3