Fluorescent indicators for Zn2+—Table 19.6

Indicator	Water-Soluble Salt	Cell-Permeant Ester	Ex/Em *	K_d for Zn²⁺ †
FluoZin-1	F24180	F24181	495/515	8 µM
FluoZin-2	NA	F24189	495/525	2 µM
FluoZin-3	F24194	F24195	494/516	15 nM **
RhodZin-3	R36350	R36351	550/575	65 nM
Newport Green DCF	N7990	N7991	505/535	1 µM
Newport Green PDX	NA	N24191	495/520	30 µM
* Excitation (Ex) and Emission (Em) maxima, in nm. † Dissociation constant of the indicator Zn²⁺ complex measured in 50 mM MOPS, pH 7.0, at 22°C. ‡ Excitation peak shifts upon binding Zn²⁺. § Emission peak shifts upon binding Zn²⁺. ** K_d determined in 135 mM NaCl, 1.1 mM EGTA, 20 mM HEPES, pH 7.4, 0–10 µM free Zn²⁺ at 22°C. NA = Not available.

For Research Use Only. Not for use in diagnostic procedures.

Fluorescent Indicators for Zn²⁺—Table 19.6