Molecular Probes heterobifunctional crosslinkers—Table 5.1 | Thermo Fisher Scientific - CL

Table 5.1 Molecular Probes heterobifunctional crosslinkers.

Cat #	Crosslinker	Reactivity
Cat #	Crosslinker	Thiol (R–SH)	Amine (R–NH₂)	*Azide (R–N₃)**	Alkyne *	Photoreactive †
S1553	succinimidyl acetylthioacetate (SATA) ‡	X	X
S1534	succinimidyl trans-4-(maleimidylmethyl) cyclohexane-1-carboxylate (SMCC) ‡	X	X
S1531	succinimidyl 3-(2-pyridyldithio)propionate (SPDP)	X	X
E2247	1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) §		X
P6317	N-((2-pyridyldithio)ethyl)-4-azidosalicylamide (PEAS; AET) ‡	X				X
A2522	4-azido-2,3,5,6-tetrafluorobenzoic acid, succinimidyl ester (ATFB, SE)		X			X
B1508	benzophenone-4-maleimide	X				X
B1526	benzophenone-4-isothiocyanate		X			X
B1577	4-benzoylbenzoic acid, succinimidyl ester		X			X
I10188	iodoacetamide azide *	X			X
I10189	iodoacetamide alkyne *	X		X
C10413	Click-iT maleimide DIBO alkyne *	X		X
A10280	azido (PEO)₄ propionic acid, succinimidyl ester *		X		X
A10279	alkyne, succinimidyl ester *		X	X
C10414	Click-iT succinimidyl ester DIBO alkyne *		X	X
* Azides react with alkynes via the copper-catalyzed azide–alkyne cycloaddition reaction, as discussed in Click Chemistry—Section 3.1 (where these products are also described). † Reacts nonspecifically with available sites upon UV illumination. ‡ These reagents contain cryptic thiols that are exposed by disulfide reduction (SPDP or PEAS) or deacetylation (SATA) and can be subsequently disulfide-coupled to other thiolated molecules or thioether-coupled to maleimides or iodoacetamides. § EDAC couples amines to carboxylic acids (Derivatization Reagents for Carboxylic Acids and Carboxamides—Section 3.4).

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