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Table 11.2 Spectral characteristics of Molecular Probes actin-selective probes.

Cat. No.Actin-Selective ProbeEx/Em *
Approximate MW
F-Actin–Selective Probes
A22281Alexa Fluor 350 phalloidin346/446 †1100
N354NBD phallacidin465/5361040
A12379Alexa Fluor 488 phalloidin495/517 †1320
F432Fluorescein phalloidin496/516 †1175
O7466Oregon Green 488 phalloidin496/520 †1180
B607BODIPY FL phallacidin505/5121125
O7465Oregon Green 514 phalloidin511/528 †1280
A22282Alexa Fluor 532 phalloidin528/555 †1350
R415Rhodamine phalloidin540/565 †1250
A22283Alexa Fluor 546 phalloidin554/570 †1800
A34055Alexa Fluor 555 phalloidin555/565 †1800
B3475BODIPY 558/568 phalloidin558/5691115
A12380Alexa Fluor 568 phalloidin578/600 †1590
A12381Alexa Fluor 594 phalloidin593/617 †1620
T7471Texas Red-X phalloidin591/608 †1490
A22284Alexa Fluor 633 phalloidin625/645 †1900
A34054Alexa Fluor 635 phalloidin633/648 †1900
B12382BODIPY 650/665 phalloidin647/6611200
A22287Alexa Fluor 647 phalloidin649/666 †1950
A22285Alexa Fluor 660 phalloidin661/689 †1750
A22286Alexa Fluor 680 phalloidin677/699 †1850
B7474Biotin-XX phalloidinNA1300
P3457PhalloidinNA790
G-Actin–Selective Probes
D12371Alexa Fluor 488 DNase I495/519>31,000
D12372Alexa Fluor 594 DNase I590/617>31,000
* Excitation (Ex) and emission (Em) maxima, in nm. Spectra of phallotoxins are either in aqueous buffer, pH 7–9 (denoted †) or in methanol. Spectra of DNase I conjugates are in aqueous buffer, pH 7–8. NA = Not applicable.

For Research Use Only. Not for use in diagnostic procedures.