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流式细胞术多参数细胞凋亡分析
由于没有哪一项单独的参数能够确定细胞程序性死亡，为了获得可靠的凋亡检测结果，建议将多种方法组合使用。 我们的多参数细胞凋亡分析试剂盒旨在从单一细胞群中选择性地从活细胞和坏死细胞中区分出凋亡细胞。 该试剂盒设计用于在一次流式细胞试验中检测多项凋亡参数，有助于早期和中期凋亡反应的区分。
| Multiparametric apoptosis analysis using the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis kit with PI. Jurkat cells (T-cell leukemia, human) were treated with 10 μM camptothecin for four hours (bottom panel) or left untreated as a control (top panel). Cells were then treated with the reagents in the kit and analyzed by flow cytometry. Note that the camptothecin-treated cells (bottom panel) have a higher percentage of apoptotic cells (indicated by “A”) than the basal level of apoptosis seen in the control cells (top panel). L = live cells, D = dead cells. |
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| 产品 | Parameter 1 | Parameter 2 | Parameter 3 | Excitation (nm) | 目录号 |
|---|---|---|---|---|---|
| Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor 488 annexin V and SYTOX Green | Membrane Asymmetry | 坏死 | -- | 488 | V13240 |
| Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, with PI | Membrane Asymmetry | 坏死 | -- | 488 | V13241 |
| FITC Annexin V/Dead Cell Apoptosis Kit, with PI | Membrane Asymmetry | 坏死 | -- | 488 | V13242 |
| Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO-1 and PI | Membrane Asymmetry | 坏死 | -- | 488 | V13243 |
| Chromatin Condensation Apoptosis Kit, with Hoechst 33342 and PI | Chromatin Condensation | 坏死 | -- | UV/488 | V13244 |
| Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO-1, and PI | Chromatin Condensation | 坏死 | -- | UV/488 | V23201 |
| PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green | Membrane Asymmetry | 坏死 | -- | 488 | V35112 |
| APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green | Membrane Asymmetry | 坏死 | -- | 488/633 | V35113 |
| Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C12 resazurin, APC annexin V, and SYTOX Green | 线粒体健康 | Membrane Asymmetry | 坏死 | 488/633 | V35114 |
| Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker Red and Alexa Fluor 488 annexin V | 线粒体健康 | Membrane Asymmetry | -- | 488 | V35116 |
| Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue™ annexin V/SYTOX AADvanced™ | Membrane Asymmetry | 坏死 | -- | 405/488 | A35136 |
| Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO-1 and 7-aminoactinomycin D | Membrane Asymmetry | 坏死 | -- | 405/488 | V35123 |
| Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant DyeCycle™ Violet and SYTOX AADvanced™ | Chromatin Condensation | 坏死 | -- | 405/488 | A35135 |
资源
流式胞内染色示意小视频—细胞因子和核内靶点检测
流式细胞术学习中心—获取流式细胞术学习资源，以轻松计划、顺利开展流式实验。
Panel Builder流式配色工具—这款简单、定制化的在线工具可助您轻松解决多色流式实验的配色难题。
5步法实验资源
不可转售。Super Bright聚合物染料由Becton, Dickinson and Company授权销售。