Amine-Reactive Dyes for Nucleic Acid Sequencing—Table 8.7

Table 8.7 Amine-reactive dyes for nucleic acid sequencing.

Cat. No.	Reactive Dye *	Ex/Em §	Handbook Location
C2210	5-FAM, SE †	494/518	Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C6164	6-FAM, SE	494/518	Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C6127	5-CR 6G, SE	525/555	Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6171MP	6-JOE, SE †	522/550	Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C2211	5-TAMRA, SE	555/580	Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6123	6-TAMRA, SE †	555/580	Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6125	5-ROX, SE	580/605	Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6126	6-ROX, SE †	580/605	Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
D2184	BODIPY FL, SE ‡	505/513	BODIPY Dye Series—Section 1.4
D6140	BODIPY FL, SSE	505/513	BODIPY Dye Series—Section 1.4
D6102	BODIPY FL-X, SE	505/513	BODIPY Dye Series—Section 1.4
D6180	BODIPY R6G, SE ‡	528/550	BODIPY Dye Series—Section 1.4
D6117	BODIPY TMR-X, SE	542/574	BODIPY Dye Series—Section 1.4
D2222	BODIPY 564/570, SE ‡	565/571	BODIPY Dye Series—Section 1.4
D2228	BODIPY 581/591, SE ‡	584/592	BODIPY Dye Series—Section 1.4
D6116	BODIPY TR-X, SE	589/617	BODIPY Dye Series—Section 1.4
* FAM = carboxyfluorescein; CR 6G = carboxyrhodamine 6G; JOE = 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein; TAMRA = carboxytetramethylrhodamine; ROX = carboxy-X-rhodamine; BODIPY = a substituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivative; SE = succinimidyl ester; SSE = water-soluble sulfosuccinimidyl ester. † These are the most widely used isomers for DNA sequencing. ‡ These BODIPY derivatives were reported to be useful for automated DNA sequencing, in part because the dyes have a minimal effect on the mobility of the fragment during electrophoresis and also exhibit well-resolved spectra with narrow bandwidths. § Excitation (Ex) and emission (Em) maxima in nm.