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Table 12.1 Molecular Probes fluorescent organelle stains.

Green-Fluorescent ProbesYellow- and Orange-
Fluorescent Probes		Red-Fluorescent ProbesBlue-Fluorescent and
Other Detectable Probes
Cat. No.ProbeCat. No.ProbeCat. No.ProbeCat. No.Probe
Probes for Mitochondria:Section 12.2
A1372Nonyl acridine orangeD2884-Di-1-ASP (DASPMI)M7512MitoTracker Red CMXRos *L6868Lucigenin †
D273DiOC6(3) ‡D426DASPEIT3168JC-1 §  
D378DiOC7(3) ‡M7510MitoTracker Orange CMTMRos *D22421JC-9 §  
M7514MitoTracker Green FM *R634Rhodamine 6GM22425MitoTracker Red FM *  
R302
R22420		Rhodamine 123T668Tetramethyl-
rhodamine,
methyl ester (TMRM)		M22426MitoTracker
Deep Red FM *		  
S7529SYTO 18 yeast
mitochondrial stain		T669Tetramethyl-
rhodamine,
ethyl ester (TMRE)		    
T3168JC-1 §      
D22421JC-9 §      
Oxidation-Sensitive Probes for Mitochondria:Section 12.2
D632Dihydrorhodamine 123M7511MitoTracker Orange CM-H2TMRos *M7513MitoTracker Red CM-H2XRos *  
Probes for Acidic Organelles Including Lysosomes:Section 12.3
L7526LysoTracker Green DND-26L12491LysoTracker Yellow HCK-123L7528LysoTracker Red DND-99H22845Hydroxy-
stilbamidine
    L12492LysoTracker Deep Red
L7525LysoTracker Blue DND-22
    R14060RedoxSensor Red CC-1 **  
pH-Sensitive Probes for Acidic Organelles:Section 12.3
L7535LysoSensor Green DND-189A1301Acridine orangeN3246Neutral redL7533LysoSensor Blue DND-167
  L7545LysoSensor Yellow/Blue DND-160 §  L7545LysoSensor Yellow/Blue DND-160 §
  L22460LysoSensor Yellow/Blue 10,000 MW dextran §  L22460LysoSensor Yellow/Blue 10,000 MW dextran §
Probes for the Endoplasmic Reticulum:Section 12.4
E34250ER-Tracker GreenD282DiIC18(3)E34251ER-Tracker RedE12353ER-Tracker Blue-White DPX
D273DiOC6(3)D384DiIC16(3)    
  R648MPRhodamine B, hexyl ester    
Probes for the Golgi Apparatus:Section 12.4
D3521
B22650		BODIPY FL C5-ceramide §  D3521
B22650		BODIPY FL C5-ceramide §  
N1154
N22651		NBD C6-ceramide  D7540
B34400		BODIPY TR ceramide  
Probes for the Nucleus: Section 12.5 includes a complete listing of SYTOX, HCS NuclearMask, Hoechst, DAPI and other nuclear stains.
* Aldehyde-fixable probe. † Chemiluminescent probe. ‡ Selective for mitochondria only at low applied concentrations (<100 nM). § Dual-emission spectrum. ** The differential distribution of the oxidized product between mitochondria and lysosomes appears to depend on the oxidation–reduction (redox) potential of the cytosol.

 

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