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Glycosidase Enzymes and Their Fluorogenic and Chromogenic Substrates—Table 10.1
Table 10.1 Glycosidase enzymes and their fluorogenic and chromogenic substrates.
| Carbohydrate (Enzyme) | Notes on Enzyme Activity | Labeled Substrate (Abs/Em of the products) * | Cat # | |
|---|---|---|---|---|
| β-D-Galactopyranoside (β-Galactosidase, E.C. 3.2.1.23) |
| Blue-fluorescent product | 4-Methylumbelliferyl (360/449)  | M1489MP |
| Green-fluorescent product | Fluorescein (490/514) | F1179 | ||
| 5-(Pentafluorobenzoylamino)-fluorescein (490/514) | P11948 | |||
| C2-Fluorescein (490/514) | A22010 | |||
| C12-Fluorescein (490/514) | D2893, I2904 ‡ | |||
| 5-Chloromethylfluorescein (490/514) | D2920 † | |||
| Red-fluorescent product | C12-Resorufin (571/585) | I2906 ‡ | ||
| DDAO (646/659) | D6488 | |||
| Resorufin (571/585) | R1159 | |||
| Chromogenic substrate | 5-Bromo-4-chloro-3-indoyl (615/NA) | X-Gal; B1690 | ||
| β-D-Glucopyranoside (β-Glucosidase, E.C. 3.2.1.21) |
| Green-fluorescent product | Fluorescein (490/514) | F2881 |
| 5-(Pentafluorobenzoylamino)fluorescein (490/514) | P11947 | |||
| β-D-Glucuronide (β-Glucuronidase, GUS; E.C. 3.2.1.31) |
| Blue-fluorescent product | 4-Methylumbelliferyl (360/449) | M1490 |
| Green-fluorescent product | Fluorescein (490/514) | F2915 | ||
| 5-(Pentafluorobenzoylamino)fluorescein (490/514) | P11949 | |||
| C12-Fluorescein (490/514) | I2908 ‡ | |||
| Chromogenic substrate | 5-Bromo-4-chloro-3-indoyl (615/NA) | X-GlcU, B1691 | ||
| * Approximate absorption (Abs) and fluorescence emission (Em) maxima of enzymatic hydrolysis product, in nm. † DetectaGene Green Gene Expression Kit. ‡ ImaGene Green or ImaGene Red Gene Expression Kit. NA = Not applicable. | ||||
仅供科研使用，不可用于诊断目的。