Fluorophore | Abs * | Em * | Succinimidyl Ester | Other | Notes |
---|
Cascade Yellow | 402 | 545 | C10164 | | - Fluorescence emission spectrum shifts to shorter wavelengths in nonpolar solvents
|
Dansyl | 335 | 518 | D6104 (X) | D21 (SC) | - Sulfonyl chloride is nonfluorescent until it reacts with amines
- Weak fluorescence in aqueous solutions
|
Dapoxyl | 395 | 601 | D10161 | D10160 (SC) | - Very low fluorescence in water
- Large Stokes shifts (up to ~200 nm)
- Large extinction coefficients of Dapoxyl derivatives in some solvents
|
NBD | 466 | 535 | S1167 (X) | C20260 (AH) F486 (AH) | - NBD amine derivatives have low fluorescence in water; emission spectra and quantum yields in other solvents are variable
|
PyMPO | 415 | 570 | S6110 | | - Fluorescence emission spectrum shifts to shorter wavelengths in nonpolar solvents
|
Pyrene | 340 | 376 | P130 P6114 (CASE) | P24 (SC) | - Forms excited-state dimers (excimers) that emit at longer wavelengths (~470 nm) than the lone excited fluorophore
- Extremely long fluorescence lifetime (can be >100 ns)
|
Absorption (Abs) and Emission (Em) maxima, in nm, are for conjugates.
(AH) = Aryl halide. (CASE) = Cysteic acid separating the dye and the SE. (SC) = Sulfonyl chloride. (X) = Aminohexanoyl spacer separating the dye and the SE. |