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Table 7.4 Fluorescent dyes successfully tested with the Image-iT FX signal enhancer.
Dyes * with potentially strong background fluorescence that is reduced with the Image-iT FX Signal Enhancer † |
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- Fluorescein
- Oregon Green 488
- Oregon Green 514
- Tetramethylrhodamine
- Texas Red
- Cascade Yellow
- Dy 565
- Dy 630
- Atto 590
| - Atto 610
- Cascade Blue
- Alexa Fluor 405
- Alexa Fluor 430
- Alexa Fluor 488
- Alexa Fluor 500
- Alexa Fluor 514
- Alexa Fluor 555
- Alexa Fluor 568
| - Alexa Fluor 594
- Alexa Fluor 610
- Alexa Fluor 633
- Alexa Fluor 635
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 680
- Alexa Fluor 700
- Alexa Fluor 750
|
Dyes * with potentially weak background fluorescence that is reduced with the Image-iT FX Signal Enhancer † |
| - Alexa Fluor 532
- Alexa Fluor 546
| - Allophycocyanin
- R-phycoerythrin
|
Background-free fluorescent dyes * that are not affected by the Image-iT FX Signal Enhancer ‡ |
- Alexa Fluor 350
- IRTM 790
- Cy3
| - Pacific Blue
- Rhodamine B
- Rhodamine Red-X
| |
* All dyes were conjugated to streptavidin and tested at 10 µg/mL. † Background staining was blocked by the Image-iT FX signal enhancer. ‡ Staining was unaffected by the Image-iT FX signal enhancer. |