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Ca2+ Indicator | Water-Soluble Salt * | Cell-Permeant Ester † | Dextran ‡ | Mode § | Kd (nM) ** | Notes |
---|---|---|---|---|---|---|
Bis-fura-2 | B6810 | Ex 340/380 |
370
| 1 | ||
BTC | B6790 | B6791 | Ex 400/480 |
7000
| 2 | |
Calcium Green-1 | C3010MP | C3011MP, C3012 | C6765, C3713, C3714 | Em 530 |
190
| 3, 4 |
Calcium Green-2 | C3730 | C3732 | Em 535 |
550
| 3, 5 | |
Calcium Green-5N | C3737 | C3739 | Em 530 |
14,000
| 3 | |
Calcium Orange | C3013 | C3015 | Em 575 |
185
| 2 | |
Calcium Crimson | C3018 | Em 615 |
185
| 2 | ||
Fluo-3 | F1240, F3715 | F1241, F1242, F14218, F14242, F23915 | Em 525 |
390
| 3, 4 | |
Fluo-4 | F14200 | F14201, F14202, F14217, F23917 | Em 520 |
345
| 3 | |
Fluo-5F | F14221 | F14222 | Em 520 |
2300
| 3 | |
Fluo-4FF | F23980 | F23981 | Em 520 |
9700
| 3 | |
Fluo-5N | F14203 | F14204 | Em 520 |
90,000
| 3 | |
Fura-2 | F1200, F6799 | F1201, F1221, F1225, F14185 | F3029 | Ex 340/380 |
145
| 2 |
Fura-4F | F14174 | F14175 | Ex 340/380 |
770
| 2 | |
Fura-6F | F14178 | Ex 340/380 |
5300
| 2 | ||
Fura-FF | F14180 | F14181 | Ex 340/380 |
5500
| 2 | |
Fura Red | F14219 | F3020, F3021 | Ex 420/480 |
140
| 2, 6 | |
Indo-1 | I1202 | I1203, I1223, I1226 | Em 405/485 |
230
| 2 | |
Mag-fluo-4 | M14205 | M14206 | Em 520 |
22,000
| 3 | |
Mag-fura-2 | M1290 | M1291, M1292 | Ex 340/380 |
25,000
| 2 | |
Mag-indo-1 | M1295 | Em 405/485 |
35,000
| 2, 7 | ||
Magnesium Green | M3733 | M3735 | Em 530 |
6000
| 3 | |
Oregon Green 488 BAPTA-1 | O6806 | O6807 | O6798 | Em 520 |
170
| 3 |
Oregon Green 488 BAPTA-2 | O6808 | O6809 | Em 520 |
580
| 3, 8 | |
Oregon Green 488 BAPTA-6F | O23990 | Em 520 |
3000
| 3 | ||
Oregon Green 488 BAPTA-5N | O6812 | Em 520 |
20,000
| 3 | ||
Quin-2 | Q23918 | Em 495 |
60
| 2, 9 | ||
Rhod-2 | R14220 | R1244, R1245MP | R34676 †† | Em 580 |
570
| 3, 10 |
Rhod-3 | R10145 | Em 580 |
570
| 3 | ||
Rhod-FF | R23983 | Em 580 |
19,000
| 3 | ||
Rhod-5N | R14207 | Em 580 |
320,000
| 3 | ||
X-rhod-1 | X14210 | Em 600 |
700
| 3 | ||
X-rhod-5F | X23984 | X23985 | Em 600 |
1600
| 3 | |
* Catalog number(s) for the cell-impermeant salt. † Catalog number(s) for the cell-permeant AM ester. ‡ Catalog number(s) for the dextran conjugates. § Measurement wavelengths, in nm, where Ex = fluorescence excitation and Em = fluorescence emission. Indicators for which a pair of wavelengths are listed have dual-wavelength, ratio-measurement capability. ** Ca2+ dissociation constant, measured in vitro at 22°C in 100 mM KCl, 10 mM MOPS, pH 7.2, unless otherwise noted. Kd values depend on temperature, ionic strength, pH and other factors, and are usually higher in situ. Because indicator dextrans are intrinsically polydisperse and have variable degrees of substitution, these values may vary; lot-specific Kd values are printed on the vial in most cases. †† High-affinity dextran conjugate. 1. Ca2+-dependent fluorescence response similar to fura-2 but ~75% greater molar absorptivity. 2. The AM ester form is fluorescent (a major potential source of error in Ca2+ measurements). 3. The AM ester form is nonfluorescent. 4. Calcium Green-1 is more fluorescent than fluo-3 in both Ca2+-bound and Ca2+-free forms. The magnitude of the Ca2+-dependent fluorescence increase is greater for fluo-3; see Fluorescent Ca2+ Indicators Excited with Visible Light—Section 19.3. 5. Larger Ca2+-dependent fluorescence increase than Calcium Green-1. 6. Can also be used in combination with fluo-3 for dual-wavelength ratio measurements, Ex = 488 nm, Em = 530/670 nm . 7. Kd determined in 100 mM KCl, 40 mM HEPES, pH 7.0 at 22°C . 8. Larger Ca2+-dependent fluorescence increase than Oregon Green 488 BAPTA-1. 9. Kd determined in 120 mM KCl, 20 mM NaCl, pH 7.05 at 37°C. 10. The Kd value for the high-affinity rhod dextran (R34676) is ~780 nM. |
仅供科研使用,不可用于诊断目的。