Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
すぐに目視で確認できれば良い場合や少量のタンパク質を検出するために高感度の染色試薬が必要な場合に備えて、ゲル内検出とメンブレン上の検出に使用できる使いやすく効率的なさまざまなタンパク質染色試薬をご提供いたします。Coomassie色素ベース染色試薬、銀染色試薬、蛍光染色試薬、タグ付き融合タンパク質染色試薬、特殊染色試薬、多重プロテオミクスタンパク質ゲル染色試薬を含む弊社の包括的な染色試薬コレクションをご覧ください。
Coomassie Staining | Silver Staining | Fluorescent Protein Staining | |
---|---|---|---|
Sensitivity | 25 ng | 0.5 ng | 0.5 ng |
Ease of use | +++ | + | + |
Mode of action | In acidic buffer conditions, coomassie dye binds to basic and hydrophobic residues of proteins, changing from dull reddish-brown to intense blue. | Silver ions interact and bind with with carboxylic acid groups ( Asp and Glu), imidazole (His), sulfhydryls (Cys), and amines (Lys). Silver ions are reduced to metallic silver, resulting in brown-black color. | Most fluorescent stains involve simply dye-binding mechanisms rather than chemical reactions that alter protein functional groups. |
Detection | Visual | Visual | Compatible imaging system |
Compatibiltity with downstream applications | MS and sequencing compatible | Certain formulations are MS compatible | Most stains are MS compatible |
Learn more | Learn more | Learn more |
ヒスチジンタグ付き(Hisタグ付き)遺伝子組換えタンパク質をポリアクリルアミドゲル内で選択的に検出
ゲル内リンタンパク質/糖タンパク質検出キットおよびメンブレン上可逆的タンパク質染色キットを含みます
For Research Use Only. Not for use in diagnostic procedures.