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Protein detection and analysis—Western Blotting
Our all-encompassing range of solutions for western blotting analysis is meticulously designed and tested to meet the highest standards and minimize time and effort. Establish a robust foundation for your western blotting experiments.
On this page:
Innovative Western Blotting Solutions
Boost your productivity with western blotting tools designed for efficient high-quality data generation.
Precast mini and midi gels
- Five distinct gel chemistries—enhance the separation of proteins with different molecular weights
- Exceptional flexibility—extensive selection of polyacrylamide percentages and well configurations, including WedgeWell format for increased sample loading capacity
- Gel performance guarantee—meticulously tested for reliable, consistent performance
Invitrogen Mini Gel Tank system
- Forward-facing well configuration—easier sample loading, monitor both gel runs simultaneously
- Less running buffer required—two separate gel chambers, only use the buffer needed for each gel
- Built to last—made of durable polycarbonate
Invitrogen Midi Gel Tank system
- Higher throughput—run up to 2 midi gels and 52 samples at the same time
- Separate chambers—use only the buffer needed for each gel
- 2-in-1 design—30 min, room temperature transfer in the same tank
Power Supplies
- Bright LCD touch screen—easy to use and monitor run progress
- Simple and intuitive Interface—enter and save custom protocols in few quick steps
- Preprogrammed methods—start gel runs and transfers in seconds
Protein Ladders
- Prestained multicolor—ready-to-load, for broad, high and low molecular weight proteins
- Fluorescent optimized options—excellent for multiplexing experiments
- Specialty—IEF, native PAGE and more
Protein Stains
- Flexible—wide selection of in-gel and on-membrane protein stains
- Comprehensive—including Coomassie dye-based, silver, fluorescent, tagged fusion protein stains and PTM protein gel stains
- Compatible—select MS, western blotting and protein sequencing compatible stains available
iBlot 3 Dry Transfer System
- Fast—transfer protein in as few as 3 minutes
- Convenient—preprogrammed settings, preassembled transfer stacks, and minimal post-transfer cleanup
- Reduced lab backlog—2 independent transfer stations allow 2 users to simultaneously transfer using the same or different conditions
Power Blotter Semi-Dry Transfer System
- Versatile—use with do-it-yourself, ready-to-build, or ready-to-use transfer stacks
- Flexible to fit your lab’s needs—2 transfer cassette options to support transfer of up to 4 mini or 2 midi gels at the same time
- Integrated power supply—seamless operation between control unit and cassette, no external power supply required as with wet tank transfer
Wet Transfer Systems
- 2-in-1 tank systems—electrophoresis and transfer in the same tank
- Conserve transfer buffer—only 220 mL for mini and 300 mL for midi gel transfers
- Robust electrode plates—for highly efficient and reliable western transfers
Invitrogen iBind Automated Western Systems
- Load and go— leverage sequential lateral flow technology with no batteries, shakers, trays, or timers required
- Flexible throughput—pick a platform that matches your throughput needs; process 1 mini or midi blot, 2 mini blots, or 6 vertically cut strips using the same or different conditions
- Reproducibility—help reduce variability introduced with more hands-on, traditional processing
Bandmate Automated Western Blot Processor
- Simple setup—prepare block, wash, and antibody solutions, load, program, and walk away
- Your protocols, your buffers—no specialized reagents or protocols needed
- Antibody recovery—recover antibody for reuse in future experiments, if desired
Primary Antibodies
- Find your match—>200,000 primary antibodies with broad species and application coverage
- Extensive testing—Advanced verification with demonstrated specificity and sensitivity
- Enhanced reproducibility—take advantage of >10,000 recombinant antibodies
Chemiluminescent Detection
If you need an everyday solution to analyze a broad range of protein expression levels, an exceptionally sensitive substrate, or a solution for quantitative western blotting, we have the right chemiluminescent substrate for you.
Fluorescent Detection
Reagents, buffers and fluorescent conjugated antibodies for optimal fluorescent western blotting analysis.
No-Stain Protein Labeling Reagent
- Fast Protein Labeling—mix, label and image gels or blots
- Flexible visualization—UV or green fluorescent light
- Total Protein Normalization—broad linear dynamic range (1–80 µg)
Secondary Antibodies for Western Blotting
- Choice—wide variety of HRP-conjugated and Alexa Fluor secondary antibodies with a range of host and target species
- Excellent multiplexing abilities—detect multiple proteins at the same time with the largest selection of Alexa Fluor dyes available
- Higher signal-to-noise ratio—up to 5.8 times higher signal-to-noise ratio with Alexa Fluor Plus
Invitrogen iBright Imaging Systems
- Smooth imaging experience with minimal effort—simple, clean touchscreen interface with logical workflows, complete with a suite of automated features
- The core imaging capabilities that you need—fluorescent blot, chemiluminescent blot, protein gel, and DNA gel imaging
- The special application capabilities that you want—image membrane stains, 2D strips, TLC plates, colorimetric blots, colony plates, perform total protein normalization and more
Precast mini and midi gels
- Five distinct gel chemistries—enhance the separation of proteins with different molecular weights
- Exceptional flexibility—extensive selection of polyacrylamide percentages and well configurations, including WedgeWell format for increased sample loading capacity
- Gel performance guarantee—meticulously tested for reliable, consistent performance
Invitrogen Mini Gel Tank system
- Forward-facing well configuration—easier sample loading, monitor both gel runs simultaneously
- Less running buffer required—two separate gel chambers, only use the buffer needed for each gel
- Built to last—made of durable polycarbonate
Invitrogen Midi Gel Tank system
- Higher throughput—run up to 2 midi gels and 52 samples at the same time
- Separate chambers—use only the buffer needed for each gel
- 2-in-1 design—30 min, room temperature transfer in the same tank
Power Supplies
- Bright LCD touch screen—easy to use and monitor run progress
- Simple and intuitive Interface—enter and save custom protocols in few quick steps
- Preprogrammed methods—start gel runs and transfers in seconds
Protein Ladders
- Prestained multicolor—ready-to-load, for broad, high and low molecular weight proteins
- Fluorescent optimized options—excellent for multiplexing experiments
- Specialty—IEF, native PAGE and more
Protein Stains
- Flexible—wide selection of in-gel and on-membrane protein stains
- Comprehensive—including Coomassie dye-based, silver, fluorescent, tagged fusion protein stains and PTM protein gel stains
- Compatible—select MS, western blotting and protein sequencing compatible stains available
iBlot 3 Dry Transfer System
- Fast—transfer protein in as few as 3 minutes
- Convenient—preprogrammed settings, preassembled transfer stacks, and minimal post-transfer cleanup
- Reduced lab backlog—2 independent transfer stations allow 2 users to simultaneously transfer using the same or different conditions
Power Blotter Semi-Dry Transfer System
- Versatile—use with do-it-yourself, ready-to-build, or ready-to-use transfer stacks
- Flexible to fit your lab’s needs—2 transfer cassette options to support transfer of up to 4 mini or 2 midi gels at the same time
- Integrated power supply—seamless operation between control unit and cassette, no external power supply required as with wet tank transfer
Wet Transfer Systems
- 2-in-1 tank systems—electrophoresis and transfer in the same tank
- Conserve transfer buffer—only 220 mL for mini and 300 mL for midi gel transfers
- Robust electrode plates—for highly efficient and reliable western transfers
Invitrogen iBind Automated Western Systems
- Load and go— leverage sequential lateral flow technology with no batteries, shakers, trays, or timers required
- Flexible throughput—pick a platform that matches your throughput needs; process 1 mini or midi blot, 2 mini blots, or 6 vertically cut strips using the same or different conditions
- Reproducibility—help reduce variability introduced with more hands-on, traditional processing
Bandmate Automated Western Blot Processor
- Simple setup—prepare block, wash, and antibody solutions, load, program, and walk away
- Your protocols, your buffers—no specialized reagents or protocols needed
- Antibody recovery—recover antibody for reuse in future experiments, if desired
Primary Antibodies
- Find your match—>200,000 primary antibodies with broad species and application coverage
- Extensive testing—Advanced verification with demonstrated specificity and sensitivity
- Enhanced reproducibility—take advantage of >10,000 recombinant antibodies
Chemiluminescent Detection
If you need an everyday solution to analyze a broad range of protein expression levels, an exceptionally sensitive substrate, or a solution for quantitative western blotting, we have the right chemiluminescent substrate for you.
Fluorescent Detection
Reagents, buffers and fluorescent conjugated antibodies for optimal fluorescent western blotting analysis.
No-Stain Protein Labeling Reagent
- Fast Protein Labeling—mix, label and image gels or blots
- Flexible visualization—UV or green fluorescent light
- Total Protein Normalization—broad linear dynamic range (1–80 µg)
Secondary Antibodies for Western Blotting
- Choice—wide variety of HRP-conjugated and Alexa Fluor secondary antibodies with a range of host and target species
- Excellent multiplexing abilities—detect multiple proteins at the same time with the largest selection of Alexa Fluor dyes available
- Higher signal-to-noise ratio—up to 5.8 times higher signal-to-noise ratio with Alexa Fluor Plus
Invitrogen iBright Imaging Systems
- Smooth imaging experience with minimal effort—simple, clean touchscreen interface with logical workflows, complete with a suite of automated features
- The core imaging capabilities that you need—fluorescent blot, chemiluminescent blot, protein gel, and DNA gel imaging
- The special application capabilities that you want—image membrane stains, 2D strips, TLC plates, colorimetric blots, colony plates, perform total protein normalization and more
Everyday essentials for western blotting success
Limit the variables in your experiments by using dependable products from one trusted source.
Speed, performance and convenience
Our gel welcome packs are cost-saving bundles that contain all you need for outstanding protein separation
Maximize your results with minimal hands-on time
SuperSignal Western Blot Chemiluminescent Substrates
Total protein normalization
Total protein normalization (TPN) for quantitative western blotting analysis offers several advantages over traditional housekeeping protein-based (HKP) normalization. One optimal protein labeling reagent for TPN is the No-Stain Protein Labeling Reagent. TPN using No-Stain Labeling Reagent exhibits good linearity of response within the concentration range typically investigated (1–80 µg of protein load). The wider dynamic range prevents the loss of valuable quantitative data due to signal saturation, a common occurrence with HKP normalization. TPN using No-Stain Labeling Reagent simplifies experimental design, helping save time and resources. Unlike HKP normalization, there is no need to verify that the expression of housekeeping proteins remains unaffected by the treatment. No-Stain Labeling Reagent is particularly well-suited for TPN due to its rapid labeling protocol, and compatibility with downstream transfer and immunoblotting. It enables sensitive and stable signal detection down to 20 ng of proteins, allowing for accurate quantitation of protein expression levels.
Multiplexed fluorescent western blotting
Multiplexed western blotting is a powerful technique that offers several advantages over traditional western blotting. Its ability to simultaneously detect and quantify multiple proteins within a single sample helps increase efficiency, improves accuracy, reduces costs, and enhances data quality. It is advantageous when investigating complex biological pathways involving proteins with diverse molecular weights. The simultaneous detection of multiple protein targets in one experiment reduces experimental variability and is an excellent choice when the sample volume is limited or when analyzing large sample sets. Fluorescent western blotting requires careful planning and experiment design. Selecting the right products from the start reduces optimization and troubleshooting time and helps ensure the success of your experiment.
Western blot learning and troubleshooting
Whether you are new to western blotting, or an experienced researcher wanting to confirm your knowledge, access our educational tips and resource materials to help with every aspect of western blot analysis.
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| Videos Discover the new ways to do western blotting with our how-to videos. |
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| Protocols Find recipes for buffers, stock solutions, and general western blot protocols. |
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| Foundation Library Tech tips, application notes, handbooks and more. |
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| Troubleshooting Overcome western blot difficulties with our troubleshooting guidance. |
Stylesheet for Classic Wide Template adjustments
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웨스턴 블로팅은 중요한 단백질 생물학 도구입니다.
단백질 검출과 분석에 적합한 제품 조합을 찾아보세요. 웨스턴 블로팅 워크플로우 전 단계에 걸친 종합적인 솔루션을 통해 최소한의 시간과 노력으로 고품질의 결과를 도출할 수 있습니다.
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겔에서 멤브레인으로 단백질을 트랜스퍼하세요
항체 및 Substrate를 통한 시각화로 단백질을 검출하세요
웨스턴 블로팅 교육 자료
웨스턴 블로팅을 처음 진행하시는 분들이나 경험 있는 숙련된 연구자분들 모두에게 유용한 웨스턴 블로팅 교육 자료를 통해 웨스턴 블로팅 분석의 모든 측면에서 도움을 얻어보세요.
혁신적인 웨스턴 블로팅 솔루션
효율적으로 고품질 데이터를 생성할 수 있도록 설계된 웨스턴 블로팅 툴로 생산성을 높여보세요.
Invitrogen Mini Gel System
- 전면부 방향 웰 구성—수월한 샘플 로딩
- 전기영동 버퍼 필요량 감소—겔 체임버가 두 개로 분리되어 있어 각 겔에 필요한 버퍼만 사용 가능
- 병렬 탱크 구성—두 겔을 동시에 시각화 가능
- 호환 가능—모든 Invitrogen 프리캐스트 미니 겔, SureCast 핸드캐스트 미니 겔과 호환
Invitrogen Midi Gel System
- 겔당 최대 26개 샘플 로딩
- 챔버가 분리되어 있어 1개 또는 2개의 겔 진행이 가능해 버퍼를 아낄 수 있음
- 실온 습식 트랜스퍼에 동일한 챔버 사용
- 다수의 겔 화학물질 사용 가능
Invitrogen iBlot 3 Western Blot Transfer System
- Rapid transfer of proteins for up to 4 gels in as few as three minutes
- Excellent transfer efficiency
- Built-in cooling provides consistent, reproducible results
Invitrogen Power Blotter 트랜스퍼장치
- 10분 안에 최대 4개의 겔 트랜스퍼
- 처리량을 높이도록 설계된 교체 가능한 블로팅 카세트
- 다양한 트랜스퍼 스택 소모품
Bandmate 자동 웨스턴 블롯 처리기
- 항체 배양 및 세척 단계 완전 자동화
- 미니 블롯의 경우 최소 3.5mL, 미디 블롯은 최소 5mL의 항체만 사용
- 1차 및 2차 항체 회수
- 1~4개의 미니 블롯 , 1~2개의 미디 블롯 동시에 처리
Invitrogen iBind 자동 웨스턴 장치
- 로딩 후 즉시 처리—배터리, 셰이커, 트레이, 타이머 없이 순차적 측방향 흐름 기술을 사용하여 장치에서 용액 처리
- 항체 절약—1차 항체 사용량 최대 80% 절감
- 재현성—블롯 처리 자동화로 블롯 사이의 일관성 개선
- 민감성—iBind 시스템은 여러 단일 클론 및 다클론 항체의 수동 방식에 비해 민감성이 높음
Invitrogen iBright 이미징 시스템
- 강력한 9.1메가픽셀(MP) 카메라—논문 품질의 이미지 캡처 가능
- 스마트 노출 포착 기술—최적의 노출 시간을 신속하게 판단
- 단순한 인터페이스와 자동 기능 패키지—최소의 노력으로 신속하게 이미지 캡처
- 멀티 애플리케이션 지원—단백질 겔, 핵산 겔, 화학발광 웨스턴 블롯, 형광 웨스턴 블롯 등
- 최대 4개의 Fluorophores 다중 분석
- 21 CFR Part 11 준수 지원
Invitrogen Mini Gel System
- 전면부 방향 웰 구성—수월한 샘플 로딩
- 전기영동 버퍼 필요량 감소—겔 체임버가 두 개로 분리되어 있어 각 겔에 필요한 버퍼만 사용 가능
- 병렬 탱크 구성—두 겔을 동시에 시각화 가능
- 호환 가능—모든 Invitrogen 프리캐스트 미니 겔, SureCast 핸드캐스트 미니 겔과 호환
Invitrogen Midi Gel System
- 겔당 최대 26개 샘플 로딩
- 챔버가 분리되어 있어 1개 또는 2개의 겔 진행이 가능해 버퍼를 아낄 수 있음
- 실온 습식 트랜스퍼에 동일한 챔버 사용
- 다수의 겔 화학물질 사용 가능
Invitrogen iBlot 3 Western Blot Transfer System
- Rapid transfer of proteins for up to 4 gels in as few as three minutes
- Excellent transfer efficiency
- Built-in cooling provides consistent, reproducible results
Invitrogen Power Blotter 트랜스퍼장치
- 10분 안에 최대 4개의 겔 트랜스퍼
- 처리량을 높이도록 설계된 교체 가능한 블로팅 카세트
- 다양한 트랜스퍼 스택 소모품
Bandmate 자동 웨스턴 블롯 처리기
- 항체 배양 및 세척 단계 완전 자동화
- 미니 블롯의 경우 최소 3.5mL, 미디 블롯은 최소 5mL의 항체만 사용
- 1차 및 2차 항체 회수
- 1~4개의 미니 블롯 , 1~2개의 미디 블롯 동시에 처리
Invitrogen iBind 자동 웨스턴 장치
- 로딩 후 즉시 처리—배터리, 셰이커, 트레이, 타이머 없이 순차적 측방향 흐름 기술을 사용하여 장치에서 용액 처리
- 항체 절약—1차 항체 사용량 최대 80% 절감
- 재현성—블롯 처리 자동화로 블롯 사이의 일관성 개선
- 민감성—iBind 시스템은 여러 단일 클론 및 다클론 항체의 수동 방식에 비해 민감성이 높음
Invitrogen iBright 이미징 시스템
- 강력한 9.1메가픽셀(MP) 카메라—논문 품질의 이미지 캡처 가능
- 스마트 노출 포착 기술—최적의 노출 시간을 신속하게 판단
- 단순한 인터페이스와 자동 기능 패키지—최소의 노력으로 신속하게 이미지 캡처
- 멀티 애플리케이션 지원—단백질 겔, 핵산 겔, 화학발광 웨스턴 블롯, 형광 웨스턴 블롯 등
- 최대 4개의 Fluorophores 다중 분석
- 21 CFR Part 11 준수 지원
시작 단계에 도움이 되는 웨스턴 블로팅 툴
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겔 전기영동과 웨스턴 블롯 분석용 제품 보기
웨스턴 블롯 솔루션 책자 다운로드
표적 단백질에 맞는 웨스턴 블롯 번들 찾기
BlotBuilder 인터랙티브 제품 선택 툴
실험 요구에 맞는 단백질 겔 찾기
단백질 겔 가이드 다운로드
손쉬운 웨스턴 블롯 문서화를 위한 iBright 이미징 시스템 알아보기
iBright 1500 이미징 시스템 책자 다운로드
적합한 화학발광 기질 찾기
화학발광 HRP 기질 가이드 다운로드
생산성을 높여줄 고산출량 웨스턴 블롯 솔루션
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For Research Use Only. Not for use in diagnostic procedures.