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Find recommended media types for your specific cell line by exploring the lists below, each of which was carefully reviewed and compiled by our internal research and development (R&D) team. Cell growth, morphology, and functional performance was confirmed in the mentioned media. For any cell lines not validated internally, information was gathered from ATCC and literature searches to verify the use of specific media types for these cell lines. Explore each table and find the culture method and media type most relevant to your cell line of interest.
Cell line | Cell type | Species | Tissue | Medium |
---|---|---|---|---|
293 | fibroblast | human | embryonic kidney | MEM and 10% FBS |
3T6 | fibroblast | mouse | embryo | DMEM and 10% FBS |
A549 | epithelial | human | lung carcinoma | Ham’s F-12K and 10% FBS |
A9 | fibroblast | mouse | connective tissue | DMEM and 10% FBS |
BALB/3T3 | fibroblast | mouse | embryo | DMEM and 10% FBS |
BHK-21 | fibroblast | hamster | kidney | GMEM and 10% FBS or MEM and 10% FBS + NEAA |
BHL-100 | epithelial | human | breast | DMEM and 10% FBS |
BT | fibroblast | bovine | turbinate cells | MEM and 10% FBS + NEAA |
Caco-2 | epithelial | human | colon adeno carcinoma | MEM and 20% FBS + NEAA |
Chang | epithelial | human | liver | BME and 10% calf serum |
CHO-K1 | epithelial | hamster | ovary | Ham’s F-12K and 10% FBS |
Clone 9 | epithelial | rat | liver | Ham’s F-12K and 10% FBS |
Clone M-3 | epithelial | mouse | melanoma | Ham’s F-10 nutrient mix, 15% horse serum and 2.5% FBS |
COS-1, COS-3, COS-7 | fibroblast | monkey | kidney | DMEM and 10% FBS |
CRFK | epithelial | cat | kidney | MEM and 10% FBS + NEAA |
CV-1 | fibroblast | monkey | kidney | MEM and 10% FBS |
D-17 | epithelial | dog | osteosarcoma | MEM and 10% FBS + NEAA |
GH1, GH3 | epithelial | rat | pituitary tumor | Ham’s F-10 nutrient mix, 15% horse serum and 2.5% FBS |
HCT-15 | epithelial | human | colorectal adenocarcinoma | RPMI 1640 and 10% FBS |
HeLa | epithelial | human | cervix carcinoma | MEM and 10% FBS + NEAA (in suspension, S-MEM) |
HT-1080 | epithelial | human | fibrosarcoma | MEM and 10% heat-inactivated FBS + NEAA |
HT-29 | epithelial | human | colon adenocarcinoma | McCoy's 5A and 10% FBS |
HUVEC | endothelial | human | umbilical cord | Ham’s F-12K with 10% FBS and 100 µg/mL heparin |
I-10 | epithelial | mouse | testicular tumor | Ham’s F-10 nutrient mix, 15% horse serum and 2.5% FBS |
JEG-3 | epithelial | human | choriocarcinoma | MEM and 10% FBS |
Jensen | fibroblast | rat | sarcoma | McCoy's 5A and 5% FBS |
L2 | epithelial | rat | lung | Ham’s F-12K and 10% FBS |
LLC-WRC 256 | epithelial | rat | carcinoma | Medium 199 and 5% horse serum |
McCoy | fibroblast | mouse | unknown | MEM and 10% FBS |
MCF7 | epithelial | human | breast adenocarcinoma | MEM and 10% FBS + NEAA and 10 µg/mL insulin |
WI-38 | epithelial | human | embryonic lung | BME and 10% FBS |
WISH | epithelial | human | amnion | BME and 10% FBS |
XC | epithelial | rat | sarcoma | MEM and 10% FBS + NEAA |
Y-1 | epithelial | mouse | tumor of adrenal | Ham’s F-10 nutrient mix, 15% horse serum and 2.5% FBS |
HCT116 | epithelial | human | colon | McCoy’s with 10% FBS |
PANC1 | epithelial | human | pancreas/ duct | DMEM with 10% FBS |
NIH3T3 | fibroblast | mouse | embryo | DMEM with 10% bovine calf serum |
A431 | epithelial | human | skin / epidermis | DMEM with 10% FBS |
3T3L1 | fibroblast | mouse | embryo | DMEM with 10% bovine calf serum |
U2OS | epithelial | human | bone | McCoys with 10% FBS |
MDAMB231 | epithelial | human | mammary gland | Leibovitz's L-15 medium with 10% FBS |
NTERA2 | epithelial | human | testis | DMEM with 15% FBS |
RAW 264.7 | monocyte / macrophage | mouse | ascites | DMEM with 10% FBS |
T47D | epithelial | human | mammary gland | RPMI 1640 with 10% FBS and 0.2 units/mL bovineinsulin (or human insulin) |
PC3 | epithelial | human | prostrate | F-12K with 10% FBS |
C2C12 | myoblast | mouse | muscle | DMEM with 10% FBS |
NCCIT | epithelial | human | mediastinum | RPMI 1640 with 10% FBS |
SKNAS | epithelial | human | brain | DMEM with 10% FBS + 0.1 nM NEAA |
L6 | myoblast | rat | skeletal muscle | DMEM with 10% FBS |
GTL16 | epithelial | human | liver | RPMI 1640 with 10% FBS |
B16F10 | spindle shaped and epithelial | mouse | skin | DMEM with 10% FBS |
C8D1A | neuronal | mouse | brain, astrocyte | DMEM with 10% FBS |
HAEC | endothelial | human | aorta | M200 with LSGS kit |
F9 | epithelial | mouse | testis | DMEM with 10% FBS |
HSkMs | myoblast | human | skeletal myoblast | DMEM with 2% horse serum |
SKOV3 | epithelial | human | ovary | McCoy’s with 10% FBS |
LNCAP | epithelial | human | prostate | RPMI 1640 with 10% FBS |
A375 | epithelial | human | skin | DMEM with 10% FBS |
H1975 | epithelial | human | lung | RPMI 1640 with 10% FBS |
SW480 | epithelial | human | colon | Leibovitz's L-15 with 10% FBS |
SKBR3 | epithelial cells | human | mammary gland | McCoy’s with 10% FBS |
RD | spindle cells and large multinucleated cells | human | muscle | DMEM with 10% FBS |
SCC15 | squamous cell carcinoma | human | tongue | 1:1 mixture of DMEM and Ham's F-12 medium containing1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/mL hydrocortisone (90%); 10% FBS |
HMEC-1 | endothelial like | human | skin | MCDB 131 medium (without L-glutamine; Cat. No.10372019) with 10 ng/mL epidermal growth factor + 1 µg/mL hydrocortisone + 10 mM glutamine + 10% FBS |
CAKI-1 | epithelial | human | kidney | McCoy's with 10% FBS |
RSC96 | neuronal | rat | neuronal Schwann cell | DMEM with 10% FBS |
OVCAR3 | epithelial | human | ovary | RPMI 1640 + insulin (0.01 mg/mL) and 20% FBS |
NMUMG | epithelial | mouse | mammary gland | DMEM with 4.5 g/L glucose and 10 µg/mL insulin (90%);FBS 10% |
AML12 | epithelial | mouse | liver | DMEM/F-12 medium + 10 µg/mL insulin + 5.5 µg/mL transferrin + 5 ng/mL selenium + 40 ng/mL dexamethasone with 10% FBS |
SKMEL-31 | epithelial | human | skin | MEM with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; FBS, 15% |
MEF-IM | fibroblast | mouse | embryonic fibroblast | DMEM with 15% FBS |
BEWO | epithelial | human | placenta | F-12K media with 10% FBS |
769P | epithelial | human | kidney | RPMI 1640 with 10% FBS |
MCF10A | epithelial | human | mammary gland | MEBM with MEGM + 100 ng/mL cholera toxin |
LOVO | epithelial | human | colon | F-12K with 10% FBS |
H1299 | epithelial | human | lung | RPMI 1640 with 10% FBS |
A673 | polygonal | human | muscle | DMEM with 10% FBS |
BT-549 | epithelial | human | mammary gland | RPMI 1640 with 10% FBS, 0.023 U/mL insulin |
H4 | epithelial | human | neuroglioma | DMEM with 10% FBS |
HACAT (Addexbio) | keratinocyte | human | skin | DMEM with 10% FBS |
Htert-RPE1 | epithelial | human | eye | DMEM/F-12 medium with 10 % FBS and 0.01 mg/mL hygromycin B |
RT4 | epithelial | human | urinary bladder | McCoy's 5A with 10% FBS |
U118MG | mixed | human | brain | DMEM with 10% FBS |
SW620 | epithelial | human | colon | Leibovitz's L-15 with 10% FBS |
786-O | epithelial | human | kidney | RPMI 1640 with 10% FBS |
MJ | lymphoblast | human | blood | IMDM with 20% FBS |
Cell line | Cell type | Species | Tissue | Medium |
---|---|---|---|---|
Colo205 | epithelial | human | colon | RPMI 1640 with 10% FBS |
MIAPACA2 | epithelial | human | pancreas | DMEM with 10% FBS + 2.5% horse serum |
KATO-III | spherical | human | stomach | IMDM with 20% FBS |
Cell line | Cell type | Species | Tissue | Medium |
---|---|---|---|---|
AtT-20 | epithelial | mouse | pituitary tumor | F-10, 15% horse serum and 2.5% FBS |
Daudi | lymphoblast | human | blood from a lymphoma patient | RPMI 1640 and 10% FBS |
H9 | lymphoblast | human | T cell lymphoma | RPMI 1640 and 20% FBS |
HL-60 | lymphoblast | human | promyelocytic leukemia | RPMI 1640 and 20% FBS |
IM-9 | lymphoblast | human | marrow from myeloma patient | RPMI 1640 and 10% FBS |
Jurkat | lymphoblast | human | lymphoma | RPMI 1640 and 10% FBS |
K-562 | lymphoblast | human | myelogenous leukemia | IMDM and 10% FBS |
KG-1 | myeloblast | human | marrow from erythroleukemia patient | IMDM and 20% FBS |
AML193 | lymphoblast | human | peripheral blood | IMDM + 0.005 mg/mL insulin + 0.005 mg/mL transferrin + 5 ng/mL GM-CSF (95%) with 5% FBS |
U937 | monocyte | human | pleural effusion | RPMI 1640 with 10% FBS |
THP1 | monocyte | human | peripheral blood | RPMI 1640 with 10% FBS and 2-mercaptoethanol 0.05 mM |
PC12 | small irregular shaped cells, floating clusters; few scattered lightly attached cells | rat | adrenal gland | RPMI 1640 with 10% heat-inactivated horse serum and 5% FBS |
RAJI | lymphoblast | human | B lymphocyte | RPMI 1640 with 10% FBS |
NK92 | lymphoblast | human | peripheral blood, natural killer | Alpha MEM (no nucleosides) + 0.2 mM Inositol + 0.1mM 2-mercaptoethanol + 0.02 mM Folic acid + 200 Units/mL IL2 + 12.5% Horse Serum + 12.5 % FBS |
P388D1 | lymphoblast | mouse | monocyte, macrophage | DMEM with 10% horse serum |
RAMOS | lymphoblast | human | B lymphocyte | RPMI 1640 with 10% FBS |
EB2 | lymphoblast | human | lymph node | RPMI 1640 with 10% FBS |
SNU5 | epithelial | human | stomach | IMDM with 20% FBS |
MOLT4 | lymphoblast | human | T lymphoblast | RPMI 1640 with 10% FBS |
JEKO1 | lymphoblast | human | peripheral blood | RPMI 1640 with 20% FBS |
KARPAS 299 | lymphoblastoid | human | lymphoma | RPMI 1640 + 2 mM glutamine + 20% FBS |
HEL 92.1.7 | erythroblast | human | bone marrow | RPMI 1640 with 10% FBS |
8A3B.6 | lymphocyte | mouse | B lymphocyte | DMEM with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; 10% FBS |
REH | lymphocyte | human | lymphoblast | RPMI 1640 with 10% FBS |
EL4 | lymphoblast | mouse | T lymphocyte | DMEM with 10% horse serum |
M1 | myeloblast | mouse | myeloid leukemia | RPMI 1640 with 10% FBS |
JM1 | lymphoblast | human | blood | IMDM with 0.05 mM 2-mercaptoethanol 10% FBS |
MV411 | lymphocyte | human | peripheral blood | IMDM with 10% FBS |
ARH77 | B lymphoblast | human | peripheral blood | RPMI 1640 with 10% FBS |
KASUMI -1 | myeloblasts | human | peripheral blood | RPMI 1640 with 10% FBS |
TF1 | lymphoblast | human | bone marrow | RPMI 1640 + 2 ng/mL recombinant human GM-CSF + 10%FBS |
Expi293 | fibroblast | human | embryo | Expi293 Expression Medium |
ExpiCHO | epithelial | hamster | ovary | ExpiCHO Expression Medium |