Protein Gel Casting Cassettes

Pour your own polyacrylamide (SDS-PAGE) mini or midi gels with our sealed empty gel cassettes. These cassettes give you the option of hand casting your own gels while enjoying the benefits of the Mini Gel Tank and SureLock systems.

	Bolt Mini Gel Cassettes	Novex Empty Cassettes
Size available	Mini Gel: 8x8cm (1.0 mm thick)	Mini gel: 8x8 cm (1.5 or 1.0 mm thick) Midi gel: 8x13 cm (1.0 mm thick)
Equipment required	Mini Gel Tank or XCell SureLock Mini-Cell	Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Combs available	10 and 12-well Wedgewell (high capacity sample well)	1, 2, 2D, 5, 10, 12, 15-well
Product benefits	Load up to 60µL per well Sealed on three sides for no gel leaking No casting stand required Special coating provides an uniform polymerization rate for a more consistent handcast gel	Sealed on three sides for no gel leaking No casting stand required Special coating provides an uniform polymerization rate for a more consistent handcast gel

Recommended maximum loading volumes

Well Type	1.0 mm thickness	1.5 mm thickness
10-well Wedgewell	60 μL
12-well Wedgewell	45 μL
1-well	700 μL
5-well	60 μL
10-well	25 µL	37 µL
12-well	20 µL
15-well	15 µL	25 µL
2D-well	400 µL
12+2* well	45 µL + 15 µL
20* well	25 µL
26* well	15 µL

* Midi Gel

Documents

Gel casting recipes

Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.

Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.

Recipes for SureCast reagents

Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.

Note: Solutions do not require degassing.

	Polyacrylamide %
Solution	4%	6%	8%	10%	12%	14%	16%	18%	20%
SureCast Acrylamide (40%)	0.8 mL	1.2 mL	1.6 mL	2.0 mL	2.4 mL	2.8 mL	3.3 mL	3.6 mL	4.0 mL
SureCast Resolving Buffer	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL
Distilled water	5.1 mL	4.7 mL	4.3 mL	3.9 mL	3.5 mL	3.1 mL	2.7 mL	2.3 mL	1.9 mL
10% SureCast APS	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL
SureCast TEMED**	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL

** Add this last and mix well just before the gel is to be poured

Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.

Solution	4%
SureCast Acrylamide (40%)	0.30 mL
SureCast Stacking Buffer	0.75 mL
Distilled water	1.92 mL
10% SureCast APS	30 µL
SureCast TEMED**	3 µL

** Add this last and mix well just before the gel is to be poured

Recipes for standalone reagents

Prepare the following stock solutions: All solutions can be stored at room temperature.

50% Acrylamide/BIS (29:1)

48.3 g Acrylamide
1.7 g Bis-acrylamide

Bring to 100 mL with water
Store up to two months in a dark glass bottle

Separating Gel Buffer (1 M Tris-HCl, pH 8.8)

Add 30.3 g Tris to 150 mL water
Adjust pH 8.8 with HCL

Bring to 250 mL with water

Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)

Add 11.4 g Tris to about 150 mL water
Adjust to pH 6.8 with HCl

Bring to 250 mL with water

Catalyst- Ammonium Persulfate (Make fresh the day of use)

100 mg Ammonium Persulfate
Bring to 2 mL in water

10% SDS

10.0g SDS

Bring to 100 mL with water

50% Sucrose

50.0 g Sucrose

Bring to 100 mL with water

Separating gels

The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.

Solution	6% Gel	8% Gel	10% Gel	12% Gel	14% Gel	16% Gel	18% Gel	20% Gel
50% Acrylamide/BIS	3.0 mL	4.0 mL	5.0 mL	6.0 mL	7.0 mL	8.0 mL	9.0 mL	10.0 mL
Separating Gel Buffer	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL
10% SDS	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL
50% Sucrose*	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL
Water	7.8 mL	6.8 mL	5.8 mL	4.8 mL	3.7 mL	2.7 mL	1.7 mL	750 µL
TEMED**	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL
Catalyst**	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL

* Optional, but recommended because it makes it easy to form a good interface between the separating gel and the overlay. If omitted, increase the amount of water added to make up for the volume of the sucrose solution (increase the water by 4.0 mL for the above tables).
** Add these last and mix well just before the gel is to be poured.

Stacking gels

Following recipe is for 4% stacking gels (12.5 mL)

Solution	4%
50% Acrylamide/BIS	1.0 mL
Stacking Gel Buffer	4.2 mL
10% SDS	125 µL
Water	6.3 mL
TEMED**	5.0 µL
Catalyst**	1.0 mL

** Add this last and mix well just before the gel is to be poured

Specifications

	Bolt Mini Gel Cassettes	Novex Empty Cassettes
Size available	Mini Gel: 8x8cm (1.0 mm thick)	Mini gel: 8x8 cm (1.5 or 1.0 mm thick) Midi gel: 8x13 cm (1.0 mm thick)
Equipment required	Mini Gel Tank or XCell SureLock Mini-Cell	Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only)
Combs available	10 and 12-well Wedgewell (high capacity sample well)	1, 2, 2D, 5, 10, 12, 15-well
Product benefits	Load up to 60µL per well Sealed on three sides for no gel leaking No casting stand required Special coating provides an uniform polymerization rate for a more consistent handcast gel	Sealed on three sides for no gel leaking No casting stand required Special coating provides an uniform polymerization rate for a more consistent handcast gel

Recommended maximum loading volumes

Well Type	1.0 mm thickness	1.5 mm thickness
10-well Wedgewell	60 μL
12-well Wedgewell	45 μL
1-well	700 μL
5-well	60 μL
10-well	25 µL	37 µL
12-well	20 µL
15-well	15 µL	25 µL
2D-well	400 µL
12+2* well	45 µL + 15 µL
20* well	25 µL
26* well	15 µL

* Midi Gel

Ordering

Reagents

Documents

Documents

Gel recipes

Gel casting recipes

Choose the polyacrylamide percentage and type of well for your application based upon the expected molecular weight of your protein of interest and the volume of your sample.

Recipes for SureCast reagents

Note: Solutions do not require degassing.

	Polyacrylamide %
Solution	4%	6%	8%	10%	12%	14%	16%	18%	20%
SureCast Acrylamide (40%)	0.8 mL	1.2 mL	1.6 mL	2.0 mL	2.4 mL	2.8 mL	3.3 mL	3.6 mL	4.0 mL
SureCast Resolving Buffer	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL	2.0 mL
Distilled water	5.1 mL	4.7 mL	4.3 mL	3.9 mL	3.5 mL	3.1 mL	2.7 mL	2.3 mL	1.9 mL
10% SureCast APS	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL	80 µL
SureCast TEMED**	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL	8 µL

** Add this last and mix well just before the gel is to be poured

Solution	4%
SureCast Acrylamide (40%)	0.30 mL
SureCast Stacking Buffer	0.75 mL
Distilled water	1.92 mL
10% SureCast APS	30 µL
SureCast TEMED**	3 µL

** Add this last and mix well just before the gel is to be poured

Recipes for standalone reagents

Prepare the following stock solutions: All solutions can be stored at room temperature.

50% Acrylamide/BIS (29:1)

48.3 g Acrylamide
1.7 g Bis-acrylamide

Bring to 100 mL with water
Store up to two months in a dark glass bottle

Separating Gel Buffer (1 M Tris-HCl, pH 8.8)

Add 30.3 g Tris to 150 mL water
Adjust pH 8.8 with HCL

Bring to 250 mL with water

Stacking Gel Buffer (0.375M Tris HCl, pH 6.8)

Add 11.4 g Tris to about 150 mL water
Adjust to pH 6.8 with HCl

Bring to 250 mL with water

Catalyst- Ammonium Persulfate (Make fresh the day of use)

100 mg Ammonium Persulfate
Bring to 2 mL in water

10% SDS

10.0g SDS

Bring to 100 mL with water

50% Sucrose

50.0 g Sucrose

Bring to 100 mL with water

Separating gels

The following recipes are for approximately 25 mL of separating gels, enough for four 1.0-mm thick mini gels. Scale volumes proportionally based on the number of gels to be cast.

Solution	6% Gel	8% Gel	10% Gel	12% Gel	14% Gel	16% Gel	18% Gel	20% Gel
50% Acrylamide/BIS	3.0 mL	4.0 mL	5.0 mL	6.0 mL	7.0 mL	8.0 mL	9.0 mL	10.0 mL
Separating Gel Buffer	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL	9.4 mL
10% SDS	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL	250 µL
50% Sucrose*	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL	4.0 mL
Water	7.8 mL	6.8 mL	5.8 mL	4.8 mL	3.7 mL	2.7 mL	1.7 mL	750 µL
TEMED**	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL	6.25 µL
Catalyst**	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL	625 µL

Stacking gels

Following recipe is for 4% stacking gels (12.5 mL)

Solution	4%
50% Acrylamide/BIS	1.0 mL
Stacking Gel Buffer	4.2 mL
10% SDS	125 µL
Water	6.3 mL
TEMED**	5.0 µL
Catalyst**	1.0 mL

** Add this last and mix well just before the gel is to be poured

For Research Use Only. Not for use in diagnostic procedures.

Bolt Mini Gel Cassettes

Novex Empty Cassettes

Recommended maximum loading volumes

Documents

Gel casting recipes

Recipes for SureCast reagents

Recipes for standalone reagents

Separating gels

Stacking gels

Bolt Mini Gel Cassettes

Novex Empty Cassettes

Recommended maximum loading volumes

Documents

Gel casting recipes

Recipes for SureCast reagents

Recipes for standalone reagents

Separating gels

Stacking gels