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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

细胞培养实验方案

Protocol Name 培养基 Supplement 细胞
Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons

Neurobasal 培养基

Hibernate-E Medium

B-27 Serum Free Supplement 
Culturing Human Neural Stem CellsStemPro NSC SFM media kit (kit includes media, growth factors, and supplement)GlutaMAX™-I 补充剂Human neural stem cells
Xeno-free Culture of Neural Stem CellsNeurobasal 培养基

B-27 Supplement, XenoFree

GlutaMAX™-I 补充剂

 
Differentiating Neural Stem Cells into Neurons and Glial Cells

StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)

Neurobasal 培养基

DMEM

B-27 Serum Free Supplement

N-2补充剂

GlutaMAX™-I 补充剂

Human neural stem cells
Differentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes

Neurobasal 培养基

Astrocyte Medium (kit includes medium and supplements)

B-27 Serum Free SupplementRat glial precursor cells
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)

DMEM

DMEM/F-12 with GlutaMax™ -I

Neurobasal 培养基

KnockOut™ 血清替代品（KSR）

N-2补充剂

B-27 Serum Free Supplement

Mouse embryonic fibroblasts
Culturing Rat Fetal Neural Stem CellsStemPro NSC SFM media kit (kit includes media, growth factors, and supplement) Rat fetal neural stem cells
Derivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)Neurobasal 培养基B-27 Serum Free Supplement 
Derivation and Culture of Cortical AstrocytesAstrocyte Medium (kit includes medium and supplements)  
Cryopreserving Neural Stem CellsStemPro NSC SFM media kit
(kit includes media, growth factors, and supplement)		  
Cryopreservation and Recovery of Mature Differentiated Neural CellsNeurobasal 培养基B-27 Serum Free Supplement 

We understand that there’s more to your experiment than culturing your cells. View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

细胞分析

Protocol Name
Cell Viability Assays for Neural Stem Cells
Markers for Characterizing Neural Subtypes
Surface Marker Analysis by Flow Cytometry
Immunocytochemistry
Intracellular Calcium Assay using Fluo-4

Molecular Characterization

Protocol Name
PCR Primers for Molecular Characterization of Neural Subtypes
RNA Isolation and cDNA Preparation from Neural Stem Cells
Characterizing Neural Cells by qPCR

转染

Protocol Name
Transfecting Neural Cells Using the Neon Transfection System
Lipid-Mediated Transfection of Human Astrocytes

细胞培养资源

 

仅用于研究用途。 Not fur human or animal therapeutic or diagnostic use.